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Volume 32, Number 12—December 2026

Dispatch

Natural Occurrence of Primate SLAM-Adapted Canine Distemper Virus, Brazil, 2018

Mizuki Tominaga, Ayumu Hyodo, Shohei Kojima, Mitsuki Onodera, Hekai Yang, Fumio Seki, Yuki Kitai, Yukiko Akahori, Hiroshi Katoh, and Makoto TakedaComments to Author 
Author affiliation: The University of Tokyo, Bunkyo-ku, Tokyo, Japan (M. Tominaga, A. Hyodo, M. Onodera, H. Yang, Y. Kitai, Y. Akahori, H. Katoh, M. Takeda); Keio University Human Biology-Microbiome-Quantum Research Center, Tokyo (S. Kojima); Okayama University Medical School, Okayama, Japan (M. Onodera); Japan Institute of Health Security, Musashimurayama, Japan (F. Seki)

Main Article

Figure 4

Quantitative analysis of SLAM-dependent cell–cell fusion in study of natural occurrence of primate SLAM-adapted canine distemper virus, Brazil, 2018. We quantified cell–cell fusion mediated by hemagglutinin (HA) proteins derived from virus strains Ac96I and NHP1 by using dual-split reporter protein (DSP) assays, as described previously (4). HA proteins were unchanged or had amino acid substitutions at positions 519 or 530. Fusion protein was derived from the Ac96I virus strain. We used Vero cells expressing canine, marmoset, human, or macaque SLAM, or parental Vero cells lacking exogenous SLAM (SLAM, –) as target cells. For each HA protein, fusion activity with canine SLAM was defined as 100%, and fusion activities for the other SLAM orthologs were expressed relative to this value. Data are shown on a logarithmic scale. Bars represent means +SDs from 3 independent experiments. Open circles are individual data points. SLAM, signaling lymphocytic activation molecule.

Figure 4. Quantitative analysis of SLAM-dependent cell–cell fusion in study of natural occurrence of primate SLAM-adapted canine distemper virus, Brazil, 2018. We quantified cell–cell fusion mediated by hemagglutinin (HA) proteins derived from virus strains Ac96I and NHP1 by using dual-split reporter protein (DSP) assays, as described previously (4). HA proteins were unchanged or had amino acid substitutions at positions 519 or 530. Fusion protein was derived from the Ac96I virus strain. We used Vero cells expressing canine, marmoset, human, or macaque SLAM, or parental Vero cells lacking exogenous SLAM (SLAM, –) as target cells. For each HA protein, fusion activity with canine SLAM was defined as 100%, and fusion activities for the other SLAM orthologs were expressed relative to this value. Data are shown on a logarithmic scale. Bars represent means +SDs from 3 independent experiments. Open circles are individual data points. SLAM, signaling lymphocytic activation molecule.

Main Article

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