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Volume 32, Number 2—February 2026

Dispatch

Genomic Analysis of Doxycycline Resistance–Associated 16S rRNA Mutations in Treponema pallidum Subspecies pallidum

George S. Long, Mackenzie Neale, Thomas Braukmann, Vanessa Tran, Nishant Singh, Vanessa Allen, Muhammad Morshed, Jessica Minion, Paul Van Caeseele, Maud Vallée, Todd Hatchette, Samir N. Patel, Raymond S.W. Tsang, and Venkata R. DuvvuriComments to Author 
Author affiliation: Public Health Ontario, Toronto, Ontario, Canada (G.S. Long, T. Braukmann, V. Tran, N. Singh, V. Allen, S.N. Patel, V.R. Duvvuri); Public Health Agency of Canada, Winnipeg, Manitoba, Canada (M. Neale, R.S.W. Tsang); University of Toronto, Toronto (T. Braukmann, V. Tran, V. Allen, S.N. Patel, V.R. Duvvuri); Sinai Health and University Health Network, Toronto (V. Allen); British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada (M. Morshed); University of British Columbia, Vancouver (M. Morshed); Roy Romanow Provincial Laboratory, Regina, Saskatchewan, Canada (J. Minion); University of Saskatchewan, Regina (J. Minion); Cadham Provincial Laboratory, Winnipeg (P. Van Caeseele); University of Manitoba, Winnipeg (P. Van Caeseele); Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Quebec, Canada (M. Vallée); Dalhousie University, Halifax, Nova Scotia, Canada (T. Hatchette); York University, Toronto (V.R. Duvvuri)

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Table 2

16S rRNA mutations related to tetracycline resistance in genomic analysis of doxycycline resistance-associated 16S rRNA mutations in Treponema pallidum subsp. pallidum*

Species Resistance mutations using E. coli (U00096.1) coordinates Species-specific coordinates Reference
Escherichia coli A964G, G1053A, C1054T, A1055G A966G, G1056A, C1057T, A1058G (8)
Cutibacterium acnes G1058C G1032C (7)
Helicobacter pylori A965G, A965G & A967C, A965G & G966T, A967C A930G, A930G & A932C, A930G & G931T, A932C (6)

*Ampersands in the resistance mutations column indicate multi-allele resistance variants.

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References
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Page created: January 16, 2026
Page updated: February 09, 2026
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