Volume 32, Number 5—May 2026
Dispatch
Clinical, Molecular, and Zoonotic Perspectives on Human Cases of Cryptosporidium sp. OTUi
Figure 1

Figure 1. Phylogenetic trees inferred by using Bayesian analysis on the basis of partial nucleotide sequences of the gp60 gene of Cryptosporidium sp. OTUi (GenBank accession no. PX525565) obtained from a woman in Denmark who had traveled to Indonesia (bold text) in a study of the clinical, molecular, and zoonotic perspectives on human cases of Cryptosporidium sp. OTUi. A) Analysis including reference sequences (n = 35) retrieved from GenBank. B) Because sequences from previous human cases in Australia (GenBank accession nos. KJ506837 and PX092402) were shorter, we used a reduced alignment, including relevant reference sequences (n = 23). For Bayesian analyses, substitution models were selected on the basis of the lowest Akaike information criterion score, using a general time reversible with invariable site plus discrete gamma model substitution. We performed Bayesian inference by using MrBayes v3.2.7 (13) with 5 million generations, 4 chains, and sampling every 1,000 generations. The first 25% of trees were discarded as burn-in. We assessed convergence was assessed by SD of split frequencies <0.01 and a potential scale reduction factor of 1.0. Posterior probabilities and bootstrap support values from maximum-likelihood and neighbor-joining analyses are shown to the right of the nodes. Scale bars indicate substitutions per site.
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