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Genetically Distinct African Swine Fever Virus Genotype II Variant in Wild Boar, Spain, 2025–2026
Carmina Gallardo

, Marina Marcet-Houben, Montserrat Agüero, Raquel Nieto, Nadia Casado, Alejandro Soler, María José Ruano, Rubén Villalba, Jovita Fernández-Pinero, Alicia Simón, Covadonga Pérez, Concepción Gómez, Cecilia García, Mireia Labrador, Jordi Argilaguet, Liani Coronado, Natàlia Majó, Pilar Aguilera-Sepulveda, Irene Aldea, Toni Gabaldón, María Belén Gómez, and Marisa Arias
Author affiliation: Centro de Investigación en Sanidad Animal, Valdeolmos, Spain (C. Gallardo, R. Nieto, N. Casado, A. Soler, J. Fernandez-Pinero, A. Simón, C. Pérez, C. Gómez, P. Aguilera-Sepulveda, I. Aldea, M. Arias); Barcelona Supercomputing Center, Barcelona, Spain (M. Marcet-Houben, C. García, M. Labrador, T. Gabaldón); Institute for Research in Biomedicine, Barcelona (M. Marcet-Houben, C. García, M. Labrador, T. Gabaldón); CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain (M. Marcet-Houben, T. Gabaldón); Laboratorio Central de Veterinaria, Algete, Spain (M. Agüero, M.J. Ruano, R. Villalba, M.B. Gómez); Unitat Mixta d’Investigació Institut de Recerca i Tecnologia Agroalimentàries–Universitat Autònoma de Barcelona en Sanitat Animal, Barcelona (J. Argilaguet, L. Coronado, N. Majó); Centre de Recerca en Sanitat Animal, Barcelona (J. Argilaguet, L. Coronado); World Organisation for Animal Health Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe, Barcelona (J. Argilaguet, L. Coronado, N. Majó); Universitat Autónoma de Barcelona, Barcelona (N. Majó); Catalan Institution for Research and Advanced Studies, Barcelona (T. Gabaldón)
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Figure 3

Figure 3. Maximum-likelihood phylogenetic tree based on complete genome sequences of ASFV genotype II from study of the variant in wild boar, Spain, 2025–2026. The analysis included 40 publicly available genomes retrieved from GenBank (accession numbers shown) and the consensus genome sequence SP25WB2611 from our study (bold; wild boar, Spain, 2025 case 1 and 2). We used IQ-TREE (http://www.iqtree.org) to infer the tree with the best-fitting nucleotide substitution model selected by ModelFinder (http://www.iqtree.org/ModelFinder) outside the variable regions. Branch support values are shown at nodes (10,000 ultrafast bootstrap replicates). Scale bar indicates nucleotide substitutions per site. ASFV, African swine fever virus.
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Page created: July 21, 2026
Page updated: August 19, 2026
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