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Volume 5, Number 2—April 1999


Neospora caninum Infection and Repeated Abortions in Humans

Eskild Petersen*Comments to Author , Morten Lebech*, Lene Jensen†, Peter Lind†, Martin Rask†, Peter Bagger‡, Camilla Björkman§, and Arvid Uggla§
Author affiliations: *Statens Serum Institut, Copenhagen, Denmark; †State Veterinary Laboratory, Copenhagen, Denmark; ‡Rigshospitalet, Copenhagen, Denmark; §Swedish University of Agricultural Sciences, Uppsala, Sweden

Main Article


Results of enzyme-linked immunosorbent assay (ELISA)a tests for Neospora caninum and of the Sabin- Feldman dye testb for Toxoplasma gondii

Sample no. ELISA mean OD Dye test
90 0.720 0
107 0.627 0
262 0.578 1:50
264 1.238 0
276 1.043 1:50
279 1.032 0
282 0.647 1:10
285 0.656 0
287 1.143 1:6250
289 0.818 0
295 0.583 0
297 0.541 0

aThe ELISA was modified from Björkman et al. (8,9). A T. gondii-negative human serum specimen was used as a presumed N. caninum-negative control, and serum specimens from experimentally N. caninum-infected pigs were used as positive controls. A low-positive serum was collected from a pig infected 11 days before sampling and a high-positive control serum was pooled from pigs infected for at least 3 weeks (10). No reaction in human sera was definitely positive.
bThe dye test was used to demonstrate antibodies to T. gondii as described (11) but using in vitro cultured T. gondii.

Main Article