Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 7, Number 1—February 2001


Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene

Susana Pedraza-Díaz*, Corinne Amar*, Gordon L. Nichols†, and Jim McLauchlin*Comments to Author 
Author affiliations: *PHLS Central Public Health Laboratory, London, UK; †PHLS Communicable Disease Surveillance Centre, London, UK

Main Article

Table 2

Cryptosporidium oocyst wall protein (COWP) gene analysis of DNA extracted from 1,680 human fecal samples

PCR amplification procedure No. of samples (%) 1 2 1 & 2 3
COWP gene fragment amplified
Unnested 1,304 (77.6) 381 917 2 4
Nesteda 296 (17.6) 81 198 7 10
COWP gene fragment not amplifiedb
Oocysts detected by microscopy 35 (2.1)
Microscopy not reconfirmedc 2 (0.1)
Oocysts not reconfirmed by microscopy 43 (2.6)

aAll samples previously negative by unnested procedure.
bBy both unnested and nested procedures.
cInsufficient material available.

Main Article