Volume 7, Number 1—February 2001
Research
Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
Table 2
Cryptosporidium oocyst wall protein (COWP) gene analysis of DNA extracted from 1,680 human fecal samples
PCR amplification procedure | No. of samples (%) | 1 | 2 | 1 & 2 | 3 |
---|---|---|---|---|---|
COWP gene fragment amplified | |||||
Unnested | 1,304 (77.6) | 381 | 917 | 2 | 4 |
Nesteda | 296 (17.6) | 81 | 198 | 7 | 10 |
COWP gene fragment not amplifiedb | |||||
Oocysts detected by microscopy | 35 (2.1) | ||||
Microscopy not reconfirmedc | 2 (0.1) | ||||
Oocysts not reconfirmed by microscopy | 43 (2.6) |
aAll samples previously negative by unnested procedure.
bBy both unnested and nested procedures.
cInsufficient material available.