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Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

Mosquito Surveillance and Polymerase Chain Reaction Detection of West Nile Virus, New York State

Dennis J. White*Comments to Author , Laura D. Kramer*, P. Bryon Backenson*, Gary Lukacik*, Geraldine Johnson*, JoAnne Oliver*, John J. Howard*, Robert G. Means*, Millicent Eidson*, Ivan Gotham*, Varuni Kulasekera†, Scott Campbell‡, and the Arbovirus Research Laboratorythe Statewide West Nile Virus Response Teams
Author affiliations: *New York State Department of Health, Albany, New York, USA; †City of New York Department of Health, New York, New York, USA; ‡Suffolk County Department of Health Services, Yaphank, New York, USA

Main Article

Table 3

Comparison of West Nile virus detection in mosquito pools positive by polymerase chain reaction (PCR) tests and isolation in Vero cells

Species No. pools (indiv) tested No. pools PCR+/no. mosquitoes tested (MIR)* PCR+ (indiv) and culture + PCR+ (indiv) and culture - PCR+ (indiv) and culture not done
Culex pipiens/restuans 3,746(130,745) 212/130,745(1.6) 61(2,555) 83(4,124) 68(2,507)
Cx. pipiens 1,119(30,818) 79/30,818(2.6) 25(630) 35(1,413) 19(698)
Cx. salinarius 501(20,236) 31/20,236(1.5) 13(486) 13(666) 5(190)
Aedes vexans 1,182(35,010) 10/35,010(0.3) 1(11) 8(252) 1(21)
Ochlerotatus
japonicus 526(7,209) 5/7,209(0.7) 0 5(36) 0
Culex spp. 232(6,466) 19/6,466(2.9) 9(296) 4(187) 6(300)
Oc. triseriatus 407(9,278) 3/9,278(0.3) 1(16) 2(51) 0
Oc. cantator 87(2,608) 1/2,608(0.4) 0 1(10) 0
Aedes/Ochlerotatus spp. 73(1,365) 1/1,365(0.7) 0 0 1(20)
Anopheles punctipennis 54(456) 1/456(2.2) 0 1(5) 0
Psorophora ferox 16(225) 1/225(4.4) 1(10) 0 0

*MIR = minimum infection ratios per 1,000 tested; indiv = individually; + = positive; - = negative.

Main Article

1E.B. Kauffman, K.A. Bernard, G.D. Ebel, P-Y. Shi, S.A. Jones, A.P. Dupuis II, J. Maffei, K.A. Ngo, D.C. Nicholas, and D. Young.

2J. Miller, City of New York Department of Health; D. Ninivaggi, Suffolk County Department of Health Services; C. Hodgeman, G. Terrillion, Nassau County Health Department; A. Huang, P. Yang-Lewis, Westchester County Health Department; M. Anand, B. Hunderfund, Rockland County Health Department; M. Smith, Orange County Health Department; J. Storm, S. Capowski, Dutchess County Health Department; D. Palen, R. Krom, G. Myers, Ulster County Health Department; S. Lukowski, Albany County Health Department; J. Zukowski, Columbia County Health Department; R. Burdick, Onondaga County Health Department; and J. Mysen, United States Military Academy, West Point, New York.

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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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