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Volume 8, Number 7—July 2002

Infection by Ralstonia Species in Cystic Fibrosis Patients: Identification of R. pickettii and R. mannitolilytica by Polymerase Chain Reaction

Tom Coenye*Comments to Author , Peter Vandamme†, and John J. LiPuma*
Author affiliations: *University of Michigan Medical School, Ann Arbor, Michigan, USA; †Ghent University, Ghent, Belgium;

Main Article


Sensitivity and specificity of polymerase chain reaction (PCR) assays for the identification of Ralstonia mannitolilytica and R. pickettii

No. of strains
Primer pair and species tested Sensitivity (%) Specificity (%) Positive Negative
R. pickettii (n=27) 89 99 24 3a
All others (n=125) 1b 124
R. mannitolilytica (n=34) 100 99 34 0
All others (n=118) 1c 117

aOf the three R. pickettii isolates that gave a false-negative reaction in this PCR test, two were identified as R. pickettii by the referring laboratory, while one was received unidentified.
bOne unidentified Ralstonia sp. isolate cross-reacted with this primer pair.
cOne R. pickettii isolate cross-reacted with this primer pair.

Main Article

Page created: July 16, 2010
Page updated: July 16, 2010
Page reviewed: July 16, 2010
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