Volume 8, Number 7—July 2002
Infection by Ralstonia Species in Cystic Fibrosis Patients: Identification of R. pickettii and R. mannitolilytica by Polymerase Chain Reaction
|No. of strains|
|Primer pair and species tested||Sensitivity (%)||Specificity (%)||Positive||Negative|
|R. pickettii (n=27)||89||99||24||3a|
|All others (n=125)||1b||124|
|R. mannitolilytica (n=34)||100||99||34||0|
|All others (n=118)||1c||117|
aOf the three R. pickettii isolates that gave a false-negative reaction in this PCR test, two were identified as R. pickettii by the referring laboratory, while one was received unidentified.
bOne unidentified Ralstonia sp. isolate cross-reacted with this primer pair.
cOne R. pickettii isolate cross-reacted with this primer pair.