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Volume 8, Number 7—July 2002

Research

Emergence of Usutu virus, an African Mosquito-Borne Flavivirus of the Japanese Encephalitis Virus Group, Central Europe

Herbert Weissenböck*, Jolanta Kolodziejek†, Angelika Url*, Helga Lussy†, Barbara Rebel-Bauder*, and Norbert Nowotny†‡Comments to Author 
Author affiliations: *Institute of Pathology and Forensic Veterinary Medicine, Vienna, Austria; †Institute of Virology, University of Veterinary Medicine, Vienna, Austria; ‡United Arab Emirates University, Al Ain, United Arab Emirates;

Main Article

Figure 1

Histology and detection of viral signals in paraffin-embedded tissue sections of birds infected with Usutu virus (USUV). A–C, Eurasian Blackbird; D–F, Great Gray Owl; A, no histologic lesions present, hematoxylin and eosin staining; B immunohistochemistry, using a polyclonal antibody to West Nile virus, shows numerous positive neurons; C, in situ hybridization with USUV-specific oligonucleotide probe shows a staining pattern comparable with that in B; D, microglial nodule within the cerebral cortex, hematoxylin and eosin staining; E, immunohistochemistry shows single positive neurons within a glial nodule; F, in situ hybridization shows several positive neurons next to a glial nodule. Original magnification, x 130 (A–C), x 200 (D–F).

Figure 1. Histology and detection of viral signals in paraffin-embedded tissue sections of birds infected with Usutu virus (USUV). A–C, Eurasian Blackbird; D–F, Great Gray Owl; A, no histologic lesions present, hematoxylin and eosin staining; B immunohistochemistry, using a polyclonal antibody to West Nile virus, shows numerous positive neurons; C, in situ hybridization with USUV-specific oligonucleotide probe shows a staining pattern comparable with that in B; D, microglial nodule within the cerebral cortex, hematoxylin and eosin staining; E, immunohistochemistry shows single positive neurons within a glial nodule; F, in situ hybridization shows several positive neurons next to a glial nodule. Original magnification, x 130 (A–C), x 200 (D–F).

Main Article

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