Volume 9, Number 12—December 2003
Dispatch
Noninvasive Method for Monitoring Pneumocystis carinii Pneumonia
Michael J. Linke*, Sandy Rebholz†, Margaret Collins†, Reiko Tanaka†, and Melanie T. Cushion*†
Table 1
Efficiencies of the real-time PCR reactionsa
| Template | Range | r2 | Slope | Amplification | Efficiency | |
|---|---|---|---|---|---|---|
| mtLSU |
0.5 to 0.0005 pgs/rx |
0.999 |
-3.220 |
2.043 |
1.043 |
|
| LH |
12.5 to 1.25 x 10-5 ngs/rx |
0.966 |
-3.570 |
1.906 |
0.906 |
|
| OS | Undiluted to 1:8 dilution | 0.973 | -3.328 | 1.997 | 0.997 | |
aPCR, polymerase chain reaction; mtLSU, mitochondrial large ribosomal subunit RNA; LH, rat lung homogenate; OS, oral swab P. carinii–specific DNA; rx, reaction.
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