Volume 10, Number 7—July 2004
Dispatch
SARS Coronavirus Detection
Table 1
Primer/probe | Primer/probe sequence | Oligonucleotide orientation | Nucleotide positionb | Tm (°C)c | |
---|---|---|---|---|---|
NS pp1a (133 bp)d |
|||||
pp1a F |
GCCgTAgTgTCAgTATCATCACC |
S |
4609–4631 |
56.6 |
|
pp1a R |
AATAggACCAATCTCTgTAAgAgCC |
A |
4741–4717 |
56.7 |
|
pp1a TM |
F-TCACTTCgTCATCAAAgACATC XT gAggAgC p |
S |
4661–4690 |
66.2 |
|
NS pp1ab (88 bp)d |
|||||
NS F |
TTTTgTTgTTTCAACTggATACCAT |
S |
14387–411 |
57.0 |
|
NS R |
GAAACTgAgACgCgAgCTATgT |
A |
14474–453 |
57.3 |
|
NS TM |
F-CATCCTgATTATgTACgACTCCTAAC XT CACgAA p |
A |
14445–413 |
64.4 |
|
Surface spike glycoprotein (79 bp)d |
|||||
SS GP F |
gAggTCTTTTATTgAggACTTgCTC |
S |
23879–903 |
57.1 |
|
SS GP R |
gCATTCgCCATATTgCTTCAT |
A |
23957–937 |
57.3 |
|
SS GP TM | F-AAgCCAgCATCAgCgAgTgTCACCTTA XT p | A | 23935–908 | 66.7 |
aAbbreviations: SARS, severe acute respiratory syndrome; S, sense, A, antisense; F, 6-carboxyfluorescein attached to 5´-terminus (FAM); T, 5-carboxytetramethylrhodamine (5-TAMRA) attached to 5-ethylamino-dThymidin; NS, nonstructural; pp1ab, polyprotein 1ab gene; Tm, melting temperature; TM, TaqMan.
bBased on AY274119 isolate TOR2.
cThermodynamic Tm. All oligonucleotides were synthesized by TIB MOLBIOL Berlin.
dAmplicon length.
Page created: January 27, 2011
Page updated: January 27, 2011
Page reviewed: January 27, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.