SARS Coronavirus Detection

Andreas Nitsche*

, Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*

Author affiliations: *Robert Koch-Institut, Berlin, Germany

Table 2

C_T values and standard deviation of serial dilutions of SARS-CoV RNA subjected to different real-time RT-PCR assays^a

GE	Real-time RT-PCR systems	Artus kit n = 4	Drosten et al. (5) n = 6	NS pp1a^b n = 7	NS pp1ab^b n = 7	SS GP ^b n = 7	NS pp1a/NS pp1ab/SS GP^b n = 6
500	LightCycler	28.69 ± 0,46	28.16 ± 0.04	29.57 ± 0.87	29.56 ± 0.18	29.47 ± 0.53	28.53 ± 0.26
	SDS7700	n.d.	27.29 ± 0.09	29.63 ± 0.94	30.92 ± 0.09	26.98 ± 0.65	26.11 ± 0.40
50	LightCycler	32.68 ± 0.26	45.00	33.85 ± 0.60	32.83 ± 0.41	32.83 ± 0.41	32.48 ± 0.69
	SDS7700	n.d.	31.62 ± 0.45	33.82 ± 0.23	34.73 ± 1.04	30.33 ± 0.26	29.29 ± 0.67
10	LightCycler	34.42 ± 0.20	45.00	36.72 ± 0.42	35.99 ± 0.55	35.33 ± 0.48	34.50 ± 0.25
	SDS7700	n.d.	38.37 ± 4.02	35.69 ± 0.35	36.94 ± 0.10	32.93 ± 0.81	32.01 ± 0.44
5	LightCycler	35.66 ± 0.40	45.00	45.00	37.61 ± 0.55	37.12 ± 0.35	37.20 ± 0.30
	SDS7700	n.d.	41.15 ± 2.17	37.31 ± 0.77	37.64 ± 0.67	34.55 ± 0.50	34.09 ± 0.24

^aC_T, threshold cycle; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; RT-PCR, reverse transcription–polymerase chain reaction; GE, genome equivalents/assay; pp1ab, nonstructural polyprotein 1ab gene; n.d., not determined.
^bSee Table 1.

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Page created: January 27, 2011
Page last updated: January 27, 2011
Page last reviewed: January 27, 2011
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