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Volume 10, Number 7—July 2004

Dispatch

SARS Coronavirus Detection

Andreas Nitsche*Comments to Author , Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*
Author affiliations: *Robert Koch-Institut, Berlin, Germany

Main Article

Table 2

CT values and standard deviation of serial dilutions of SARS-CoV RNA subjected to different real-time RT-PCR assaysa

GE Real-time RT-PCR systems Artus kit
n = 4 Drosten et al. (5)
n = 6 NS pp1ab
n = 7 NS pp1abb
n = 7 SS GP b
n = 7 NS pp1a/NS pp1ab/SS GPb
n = 6
500
 LightCycler
 28.69 ± 0,46
 28.16 ± 0.04
 29.57 ± 0.87
 29.56 ± 0.18
 29.47 ± 0.53
 28.53 ± 0.26
SDS7700
 n.d.
 27.29 ± 0.09
 29.63 ± 0.94
 30.92 ± 0.09
 26.98 ± 0.65
 26.11 ± 0.40
50
 LightCycler
 32.68 ± 0.26
 45.00
 33.85 ± 0.60
 32.83 ± 0.41
 32.83 ± 0.41
 32.48 ± 0.69
SDS7700
 n.d.
 31.62 ± 0.45
 33.82 ± 0.23
 34.73 ± 1.04
 30.33 ± 0.26
 29.29 ± 0.67
10
 LightCycler
 34.42 ± 0.20
 45.00
 36.72 ± 0.42
 35.99 ± 0.55
 35.33 ± 0.48
 34.50 ± 0.25
SDS7700
 n.d.
 38.37 ± 4.02
 35.69 ± 0.35
 36.94 ± 0.10
 32.93 ± 0.81
 32.01 ± 0.44
5
 LightCycler
 35.66 ± 0.40
 45.00
 45.00
 37.61 ± 0.55
 37.12 ± 0.35
 37.20 ± 0.30
SDS7700 n.d. 41.15 ± 2.17 37.31 ± 0.77 37.64 ± 0.67 34.55 ± 0.50 34.09 ± 0.24

aCT, threshold cycle; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; RT-PCR, reverse transcription–polymerase chain reaction; GE, genome equivalents/assay; pp1ab, nonstructural polyprotein 1ab gene; n.d., not determined.
bSee Table 1.

Main Article

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