Volume 10, Number 7—July 2004
Dispatch
SARS Coronavirus Detection
Table 2
GE | Real-time RT-PCR systems | Artus kit n = 4 | Drosten et al. (5) n = 6 | NS pp1ab n = 7 | NS pp1abb n = 7 | SS GP b n = 7 | NS pp1a/NS pp1ab/SS GPb n = 6 | |
---|---|---|---|---|---|---|---|---|
500 |
LightCycler |
28.69 ± 0,46 |
28.16 ± 0.04 |
29.57 ± 0.87 |
29.56 ± 0.18 |
29.47 ± 0.53 |
28.53 ± 0.26 |
|
SDS7700 |
n.d. |
27.29 ± 0.09 |
29.63 ± 0.94 |
30.92 ± 0.09 |
26.98 ± 0.65 |
26.11 ± 0.40 |
||
50 |
LightCycler |
32.68 ± 0.26 |
45.00 |
33.85 ± 0.60 |
32.83 ± 0.41 |
32.83 ± 0.41 |
32.48 ± 0.69 |
|
SDS7700 |
n.d. |
31.62 ± 0.45 |
33.82 ± 0.23 |
34.73 ± 1.04 |
30.33 ± 0.26 |
29.29 ± 0.67 |
||
10 |
LightCycler |
34.42 ± 0.20 |
45.00 |
36.72 ± 0.42 |
35.99 ± 0.55 |
35.33 ± 0.48 |
34.50 ± 0.25 |
|
SDS7700 |
n.d. |
38.37 ± 4.02 |
35.69 ± 0.35 |
36.94 ± 0.10 |
32.93 ± 0.81 |
32.01 ± 0.44 |
||
5 |
LightCycler |
35.66 ± 0.40 |
45.00 |
45.00 |
37.61 ± 0.55 |
37.12 ± 0.35 |
37.20 ± 0.30 |
|
SDS7700 | n.d. | 41.15 ± 2.17 | 37.31 ± 0.77 | 37.64 ± 0.67 | 34.55 ± 0.50 | 34.09 ± 0.24 |
aCT, threshold cycle; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; RT-PCR, reverse transcription–polymerase chain reaction; GE, genome equivalents/assay; pp1ab, nonstructural polyprotein 1ab gene; n.d., not determined.
bSee Table 1.
References
- Peiris JS, Lai ST, Poon LL, Guan Y, Yam LY, Lim W, Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet. 2003;361:1319–25. DOIPubMedGoogle Scholar
- Poutanen SM, Low DE, Henry B, Finkelstein S, Rose D, Green K, Identification of severe acute respiratory syndrome in Canada. N Engl J Med. 2003;348:1995–2005. DOIPubMedGoogle Scholar
- Tsang KW, Ho PL, Ooi GC, Yee WK, Wang T, Chan-Yeung M, A cluster of cases of severe acute respiratory syndrome in Hong Kong. N Engl J Med. 2003;348:1977–85. DOIPubMedGoogle Scholar
- Acute respiratory syndrome. China, Hong Kong Special Administrative Region of China, and Viet Nam. Wkly Epidemiol Rec. 2003;78:73–4.PubMedGoogle Scholar
- Drosten C, Günther S, Preiser W, van der Werf S, Brodt HR, Becker S, Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med. 2003;348:1967–76. DOIPubMedGoogle Scholar
- Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret; Emery S, et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med. 2003;348:1953–66. DOIPubMedGoogle Scholar
- World Health Organization. Case definition for surveillance of severe acute respiratory syndrome SARS. [cited 2003 May 1]. Available from: http://www.who.int/csr/sars/casedefinition/en/
- Poon LL, Wong OK, Chan KH, Luk W, Yuen KY, Peiris JS, Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). Clin Chem. 2003;49:953–5. DOIPubMedGoogle Scholar
- Pratelli A, Martella V, Elia G, Decaro N, Aliberti A, Buonavoglia D, Variation of the sequence in the gene encoding for transmembrane protein M of canine coronavirus (CCV). Mol Cell Probes. 2001;15:229–33. DOIPubMedGoogle Scholar
- Vaughn EM, Halbur PG, Paul PS. Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, and 3-1 genes. J Virol. 1995;69:3176–84.PubMedGoogle Scholar
- Ruan YJ, Wie CL, Ee AL, Vega VB, Thoreau H, Su ST, Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet. 2003;361:1779–85. DOIPubMedGoogle Scholar
- Holmes KV, Lai MMC. Coronaviridae: The viruses and their replication. In: Fields BN, Knipe DM, Howley PRM, editors. Fields virology. 3rd ed. New York: Lippincott Williams & Wilkins; 2001. p. 1075–94.
- Chinese SARS Molecular Epidemiology Consortium. Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China. Science. 2004;303:1666–9. Epub 2004 Jan 29. DOIPubMedGoogle Scholar
- People’s daily edited by ProMED-mail. SARS worldwide. ProMed January 2, 2004. Accessed at: http://www.promedmail.org, archive number: 20040102.0015. 19991013.1826.
- Wood L. questions about comparative genomics of SARS coronavirus isolates. Lancet. 2003;362:578–9. DOIPubMedGoogle Scholar
- Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. A guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 2004;313:856–62. DOIPubMedGoogle Scholar
- Kondro W. Canadian officials watch SARS-like mystery bug. Lancet. 2003;362:714. DOIPubMedGoogle Scholar
Page created: January 27, 2011
Page updated: January 27, 2011
Page reviewed: January 27, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.