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Volume 14, Number 5—May 2008
Dispatch

Naturally Acquired Human Plasmodium knowlesi Infection, Singapore

Lee Ching Ng§Comments to Author , Eng Eong Ooi†, Cheng Chuan Lee*, Piao Jarrod Lee‡, Natalie Woon Hui Tan*Comments to Author , Sze Wong Pei§, Tian Ming Tu‡, Jin Phang Loh†, and Yee Sin Leo*
Author affiliations: *Tan Tock Seng Hospital, Singapore; †DSO National Laboratories, Singapore; ‡Singapore Armed Forces Headquarters Medical Corps, Singapore; §Environmental Health Institute, Singapore;

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Table

Primers used for the PCR investigation of the clinical sample from Singapore*

Primers Forward Sequence (5′ → 3’) Reverse Sequence (5′ → 3’) Results
Nest 1, genus specific primers
Genus specific (11)
 rPLU 1
 TCA AAG AAT AAG CCA TGC AAG TGA
 rPLU 2
 TAC CCT GTT GTT GCC TTA AAC TCC
 +
Nest 2, genus- and species-specific primers
Genus specific (11) rPLU 3 TTT TTA TAA GGA TAA CTA CGG AAA AGC TGT rPLU 4 TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC +
Plasmodium knowlesi 
specific (5) Pmk8 GTT AGC GAG AGC CAC AAA AAA GCG AAT Pmkr9 ACT CAA AGT AAC AAA ATC TTC CGT A +
P. vivax specific (11) rVIV1 CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC rVIV2 ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA –
P. falciparum specific (11) rFAL1 TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT rFAL2 ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC –
P. malariae specific (11) rMAL1 ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC rMAL2 AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA –
P. ovale specific (11) rOVA1 ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA rOVA2 GGA AAA GGA CAC ATT AAT TGT ATC CTA GTG –

*PCR was carried out at the Environmental Health Institute and cycling conditions used were as described in the references shown in parentheses.
+, positive; –,negative.

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