Volume 16, Number 6—June 2010
Research
Astrovirus Encephalitis in Boy with X-linked Agammaglobulinemia
Phenix-Lan Quan, Thor A. Wagner, Thomas Briese, Troy R. Torgerson, Mady Hornig, Alla Tashmukhamedova, Cadhla Firth, Gustavo Palacios, Ada Baisre-De-Leon, Christopher D. Paddock, Stephen K. Hutchison, Michael Egholm, Sherif R. Zaki, James E. Goldman, Hans D. Ochs, and W. Ian Lipkin
Table 6
Primers used in cloning and sequencing the human astrovirus Puget Sound genome
| Sequence (5′ → 3′) | Position |
|---|---|
| TCATGGAGCGCTCATACAAG GTGTAAGCGAAGCCAAAAGC | 38–57 815–796 |
| GATTGGGTTGAGGTTGATGC* TCCAGTGGTGGCTTGATGTA | 340–359 1797–1778 |
| GAAGTGGGATGGTGGAGTTG TCCAGAAATCCGATTCAACC | 666–685 1314–1295 |
| TGGAGCAGTTGTTGGTGAAA TTCTCAAGGTCTATCTCCCTTTGT | 1215–1234 1877–1854 |
| TACATCAAGCCACCACTGGA* TCCGCCTCTCTAAGCACTTC | 1778–1797 2159–2140 |
| TACATCAAGCCACCACTGGA TTGGATTGACTCCCTCAAGC | 1778–1797 2533–2514 |
| ATCACCACAAGAGGCGTAGG* CCAGTAACTGCTGATGGACCAACAA | 2039–2058 4649–4625 |
| CGTGATTTGCAGGAATACCA CCCATACGTGTCAGGGTTCT | 2446–2465 3343–3324 |
| TCTGGAGAGAGGCCTGATGT TCTCTCTTGACCCACATCCC | 3159–3178 3865–3846 |
| CCTCTGGGCAAATATCCACA TAGGGATATGCGGAAAACAGA | 3625–3644 4671–4651 |
| GTTTGTGGCGCTTGAAAAGT TTGCGTGGAAGAAAGTGTTG | 4583–4602 5365–5345 |
| TTGTTGGTCCATCAGCAGTTACTGG* AACTTTTGCAACCAAGCCAG | 4 625–4649 5175–5156 |
| CTGGCTTGGTTGCAAAAGTT GAGTATTGTGCCCGCAAAGT | 5156–5175 6041–6022 |
| CGGCTACACCAGCCTACATT CAGCACCATCAGACACATGG | 5833–5852 6442–6423 |
*Primer pairs designed for amplification between ultra–high-throughput sequencing contiguous fragments (Figure 2); primer pairs without asterisk were applied for resequencing of the draft genome.
Page created: June 08, 2011
Page updated: June 08, 2011
Page reviewed: June 08, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.