Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 6, Number 3—June 2000
Research

Rhinosporidium seeberi: A Human Pathogen from a Novel Group of Aquatic Protistan Parasites

David N. Fredricks*†Comments to Author , Jennifer A. Jolley*, Paul W. Lepp*, Jon C. Kosek†, and David A. Relman*†
Author affiliations: *Stanford University, Stanford, California, USA; and †Veterans Affairs, Palo Alto Health Care System, Palo Alto, California, USA

Main Article

Figure 4

An oligonucleotide probe complementary to a unique region of the Rhinosporidium seeberi 18S rRNA sequence localizes to visible organisms in human nasal tissue by using fluorescence in situ hybridization. Confocal micrographs at 200X magnification. The Cy-5-labeled Rhinosporidium probe (blue) hybridizes to spherical R. seeberi trophocytes (A) and a sporangium with endospores (B). A control probe consisting of the complement of the Rhinosporidium probe labeled with Cy-5 does not hybridize to the t

Figure 4. An oligonucleotide probe complementary to a unique region of the Rhinosporidium seeberi 18S rRNA sequence localizes to visible organisms in human nasal tissue by using fluorescence in situ hybridization. Confocal micrographs at 200X magnification. The Cy-5-labeled Rhinosporidium probe (blue) hybridizes to spherical R. seeberi trophocytes (A) and a sporangium with endospores (B). A control probe consisting of the complement of the Rhinosporidium probe labeled with Cy-5 does not hybridize to the trophocytes (C) or a sporangium with endospores (D). Images were collected in two wave-length channels: the first for Texas Red (red) or FITC (green) displays tissue architecture through autofluorescence, and the second channel for Cy-5 (blue pseudocolor) displays the probe signal.

Main Article

Page created: December 16, 2010
Page updated: December 16, 2010
Page reviewed: December 16, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external