Volume 8, Number 7—July 2002
Research
Infection by Ralstonia Species in Cystic Fibrosis Patients: Identification of R. pickettii and R. mannitolilytica by Polymerase Chain Reaction
Tom Coenye*
, Peter Vandamme†, and John J. LiPuma*
, Peter Vandamme†, and John J. LiPuma*
Table
Sensitivity and specificity of polymerase chain reaction (PCR) assays for the identification of Ralstonia mannitolilytica and R. pickettii
| No. of strains | ||||
|---|---|---|---|---|
| Primer pair and species tested | Sensitivity (%) | Specificity (%) | Positive | Negative |
| Rp-F1/Rp-R1 | ||||
| R. pickettii (n=27) | 89 | 99 | 24 | 3a |
| All others (n=125) | 1b | 124 | ||
| Rm-F1/Rm-R1 | ||||
| R. mannitolilytica (n=34) | 100 | 99 | 34 | 0 |
| All others (n=118) | 1c | 117 | ||
aOf the three R. pickettii isolates that gave a false-negative reaction in this PCR test, two were identified as R. pickettii by the referring laboratory, while one was received unidentified.
bOne unidentified Ralstonia sp. isolate cross-reacted with this primer pair.
cOne R. pickettii isolate cross-reacted with this primer pair.
Page created: July 16, 2010
Page updated: July 16, 2010
Page reviewed: July 16, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.