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Volume 11, Number 12—December 2005
Dispatch

Passatempo Virus, a Vaccinia Virus Strain, Brazil

Juliana A. Leite*, Betânia P. Drumond*, Giliane S. Trindade*, Zélia I.P. Lobato*, Flávio G. da Fonseca†, João R. dos Santos*, Marieta C. Madureira‡, Maria I.M.C. Guedes*, Jaqueline M.S. Ferreira*, Cláudio A. Bonjardim, Paulo C.P. Ferreira*, and Erna G. Kroon*Comments to Author 
Author affiliations: *Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; †Centro de Pesquisas René Rachou–Fundação Instituto Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil; ‡Instituto Mineiro de Agropecuária, Belo Horizonte, Minas Gerais, Brazil

Main Article

Figure A2

Electron micrographs of cells infected with Passatempo virus. Vero cells were infected with Passatempo virus at a multiplicity of infection of 5 and fixed by OsO4, 24 hours after inoculation. 1, typical orthopoxvirus "brick-shaped" morphologic pattern, with biconcave core and outer membranes were observed in cell-associated enveloped virus (CEV); 2, intracelullar mature virions (IMV) were visualized in the cytoplasm of infected cells within virosomes with the presence of spheric immature virus (

Figure A2. Electron micrographs of cells infected with Passatempo virus. Vero cells were infected with Passatempo virus at a multiplicity of infection of 5 and fixed by OsO4, 24 hours after inoculation. 1, typical orthopoxvirus "brick-shaped" morphologic pattern, with biconcave core and outer membranes were observed in cell-associated enveloped virus (CEV); 2, intracelullar mature virions (IMV) were visualized in the cytoplasm of infected cells within virosomes with the presence of spheric immature virus (IV). No A-type inclusion body (ATI) was visualized in infected cells. N, nucleus. Bars, 500 nm.

Main Article

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