Volume 11, Number 5—May 2005
Research
Low Diversity of Alkhurma Hemorrhagic Fever Virus, Saudi Arabia, 1994–1999
Rémi N. Charrel*
, Ali Mohamed Zaki†, Mazen Fakeeh†, Amany Ibrahim Yousef†, Reine de Chesse*, Houssam Attoui*, and Xavier de Lamballerie*
, Ali Mohamed Zaki†, Mazen Fakeeh†, Amany Ibrahim Yousef†, Reine de Chesse*, Houssam Attoui*, and Xavier de Lamballerie*
Table 2
Positions of primers used for PCR amplification and sequencing of AHFV genome and resulting sequences*
| Primer name | Sequence | Position, per AHFV prototype sequence | PCR product size (bp) | Sequence used for analysis† |
|---|---|---|---|---|
| ALK-ES | GGATATGTGTATGATGTCAATAA | 1342–1364 | 742 | 699 |
| ALK-ER | GCTGCAGTTCAACGAAACCT | 2083–2064 | ||
| ALK-NS3S | CAATGAAGCTTATGTTAGTAGC | 5064–5085 | 757 | 713 |
| ALK-NS3R | CACAAAATCTGGCTTCTCTTCT | 5820–5799 | ||
| ALK-NS5S | AGCAAATCCTGCGTTATCTGA | 9308–9328 | 723 | 685 |
| ALK-NS5R | GTCCCTGCGGGACCCAG | 10030–10014 |
*AHFV, Alkhurma hemorrhagic fever virus; PCR, polymerase chain reaction.
†Primers excluded.
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