Volume 11, Number 9—September 2005
Research
Molecular Epidemiology of SARS-associated Coronavirus, Beijing
Wei Liu*, Fang Tang*, Arnaud Fontanet†, Lin Zhan*, Tian-Bao Wang‡, Pan-He Zhang*, Yi-He Luan‡, Chao-Yang Cao‡, Qiu-Min Zhao*, Xiao-Ming Wu*, Zhong-Tao Xin§, Shu-Qing Zuo*, Laurence Baril†, Astrid Vabret¶, Yi-Ming Shao#, Hong Yang*, and Wu-Chun Cao*
Table 1
Primers used for nested polymerase chain reaction and sequencing
| Position | Amplification region* | Primer sets (starting from 5´) | |
|---|---|---|---|
| 17564 | 17440–18281 | Forward | ACGTCTATATTGGCGATCCT TGTGCAGACTTATGAAAACAATA |
| Reverse | GTTTTGCATTAACTCTGGTG GTTAGTACCCACAGCATCTCTAGT | ||
| 21721 | 21585–22304 | Forward | GATGATGTTCAAGCTCCTAATTAC CTTAACAGAGCATTTGAGTTCAG |
| Reverse | CAACATACTTCATCTATGAGGGG TGTACCATTTTCATCATACTTGAG | ||
| 22222 | 22177–22874 | Forward | AGATGTAGTTCGTGATCTACCTTC TTAATGGCCAATAACAATTAAGA |
| Reverse | CAAATTTTAGAGCCATTCTTACAG GGAGAAAGGCACATTAGATATGTC | ||
| 23823 | 23455–24263 | Forward | CGACACTTCTTATGAGTGCG ATGCAGTTGATGTTGTTGTAAG |
| Reverse | GCATTTGTGCTAGTTACCATACAG TGATGTTGTTGTAAGTGATTCTTG | ||
| 27827 | 27449–28270 | Forward | CCATCAGGAACATACGAGG GACCACTATTGGTGTTGATTG |
| Reverse | TAGCACACACTTTGCTTTTG CAGTATTATTGGGTAAACCTTGG | ||
*The nucleotide position was given with TOR2 as the reference strain (accession no. NC004718).
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