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Volume 12, Number 4—April 2006
Research

Identifying Influenza Viruses with Resequencing Microarrays

Zheng Wang*†, Luke T. Daum‡, Gary J. Vora*, David Metzgar§, Elizabeth A. Walter¶, Linda C. Canas‡, Anthony P. Malanoski*, Baochuan Lin*, and David A. Stenger*Comments to Author 
Author affiliations: *Naval Research Laboratory, Washington, DC, USA; †NOVA Research Inc., Alexandria, Virginia, USA; ‡Air Force Institute for Operational Health, Brooks City Base, San Antonio, Texas, USA; §Naval Health Research Center, San Diego, California, USA; ¶Lackland Air Force Base, San Antonio, Texas, USA

Main Article

Figure 1

Hybridization images of the respiratory pathogen microarray (RPM) version 1 prototype regions for 3 influenza virus isolates and trivalent FluMist vaccine. A) A/H1N1, B) A/H3N2, C) influenza B, and D) trivalent FluMist vaccine. In A, B, and C, only the influenza-specific tiled prototype regions of RPM version 1 are shown. Hybridization-positive identifications are shown on the right. In D, the image of the entire RPM version when hybridized with FluMist vaccine is shown. The single influenza pro

Figure 1. Hybridization images of the respiratory pathogen microarray (RPM) version 1 prototype regions for 3 influenza virus isolates and trivalent FluMist vaccine. A) A/H1N1, B) A/H3N2, C) influenza B, and D) trivalent FluMist vaccine. In A, B, and C, only the influenza-specific tiled prototype regions of RPM version 1 are shown. Hybridization-positive identifications are shown on the right. In D, the image of the entire RPM version when hybridized with FluMist vaccine is shown. The single influenza prototype region that was hybridization negative is denoted on the right. E) Magnification of a portion of profile B showing an example of the primary sequence data generated by the hybridization of randomly amplified targets to the RPM version 1 HA3 probe set. The primary sequence generated can be read from left to right. HA, hemagglutinin; NA, neuraminidase; IQEX, internal positive hybridization control (Affymetrix); M, matrix.

Main Article

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