Volume 13, Number 6—June 2007
Research
Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
Kanako Watanabe*†, Masayasu Oie*, Masaya Higuchi*, Makoto Nishikawa†, and Masahiro Fujii*
Table 2
Comparisons of nucleotide and amino acid sequences among NII561-2000, HPeV-1, HPeV-2, HPeV-3, HPeV-4, and HPeV-5*
| Sequence | % Nucleotide similarity (% amino acid similarity) with NII561-2000 |
||||
|---|---|---|---|---|---|
| HPeV-1† | HPeV-2 | HPeV-3 | HPeV-4 | HPeV-5 | |
| 5′UTR | 85.3 | 87.3 | 86.9 | 88.5 | 86.9 |
| VP0 | 73.6 (78.2) | 76.0 (80.9) | 70.6 (72.3) | 72.6 (81.0) | 73.1 (77.2) |
| VP3 | 74.4 (84.7) | 73.0 (80.2) | 67.0 (74.1) | 70.9 (78.0) | 68.7 (73.1) |
| VP1 | 71.7n(80.3) | 67.3 (72.2) | 71.1 (75.3) | 70.0 (71.9) | 66.2 (70.2) |
| 2A | 80.8 (90.7) | 77.1 (88.0) | 81.0 (85.3) | 77.3 (91.2) | 76.2 (88.7) |
| 2B | 78.6 (97.5) | 77.8 (97.5) | 82.0 (97.5) | 79.8 (97.5) | 78.7 (99.2) |
| 2C | 84.7 (97.3) | 76.4 (86.9) | 79.2 (92.7) | 78.7 (92.4) | 79.2 (93.6) |
| 3A | 85.7 (96.6) | 77.4 (88.0) | 78.0 (87.2) | 75.5 (89.7) | 78.6 (83.8) |
| 3B | 81.4 (80.0) | 72.9 (80.0) | 73.3 (75.0) | 66.7 (85.0) | 70.0 (85.0) |
| 3C | 84.2 (99.0) | 82.7 (99.0) | 82.3 (98.0) | 82.5 (98.0) | 80.5 (99.0) |
| 3D | 83.2 (95.9) | 83.7 (95.5) | 83.9 (95.7) | 84.2 (96.8) | 87.2 (97.0) |
| 3′UTR | 81.6 | 88.5 | 83.9 | 84.9 | 93.2 |
| ORF | 79.5 (90.7) | 77.1 (87.3) | 76.7 (85.9) | 77.1 (88.2) | 77.0 (86.9) |
*HPeV, human parechovirus; UTR, untranslated region; ORF, open reading frame.
†The HPeV strains used were Harris strain for HPeV-1, Williamson strain for HPeV-2, A308/99 for HPeV-3, K2511876–02 for HPeV-4, and CT86–6760 for HPeV-5.
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