Volume 13, Number 9—September 2007
Dispatch
Anaplasma platys in Dogs, Chile
Table 1
Ehrlichia/Anaplasma spp. PCR primers used in this study
Ehrlichia/Anaplasma spp. (primer type) | Primer | Primer sequence (5′→3′) | Region | Reference |
---|---|---|---|---|
Ehrlichia spp., A. phagocytophilum, E. canis, E. chaffeensis, E. ewingii, A. platys (outer) | EHR-OUT1 | CTGGCGGCAAGCCTAACACATGCCAACAT | 16S rRNA | (5) |
EHR-OUT2 | GCTCGTTGCGGGACTTAACCCAACATCTCACGAC | 16S rRNA | (5) | |
Ehrlichia spp. (inner) | GE2F | GTTAGTGGCATACGGGTGAAT | 16S rRNA | (5) |
EHRL3-IP2 | TCATCTAATAGCGATAAATC | 16S rRNA | (5) | |
A. phagocytophilum (inner) | ge9f | AACGGATTATTCTTTATAGCTTGCT | 16S rRNA | (6) |
ge2 | GGCAGTATTAAAAGCAGCTCCAGG | 16S rRNA | (6) | |
E. canis, E. chaffeensis, E. ewingii (inner) | HE3-R | CTTCTATAGGTACCGTCATTATCTTCCCTAT | 16S rRNA | (5) |
E. canis (inner) | E. canis | CAATTATTTATAGCCTCTGGCTATAGGAA | 16S rRNA | (5) |
E. chaffeensis (inner) | E. chaffeensis | CAATTGCTTATAACCTTTTGGTTATAAATA | 16S rRNA | (5) |
E. ewingii (inner) | E. ewingii | CAATTCCTAAATAGTCTCTGACTATT | 16S rRNA | (5) |
E. equi (inner) | E. equi-3-IP2 | GTCGAACGGATTATTCTTTATAGCTTG | 16S rRNA | (5) |
E. platys (inner) | EHRL3-IP2 | TCATCTAATAGCGATAAATC | 16S rRNA | (5,7) |
E. platys | GATTTTTGTCGTAGCTTGCTA | 16S rRNA | (7) | |
E. platys (outer) | EEgro1F | GAGTTCGACGGTAAGAAGTTCA | groESL | (8) |
EEgro2R | CAGCGTCGTTCTTACTAGGAAC | groESL | (8) | |
A. platys (inner) | SQ3F | ATTAGCAAGCCTTATGGGTC | groESL | (9) |
SQ5F | TCAGTGTGTGAAGGAAGTTG | groESL | (9) | |
SQ4R | CTTTAGGCTATCAAGAGATG | groESL | (9) | |
SQ6R | TGCTTCCTATGTTCTTATCG | groESL | (9) |
References
- González-Acuña D, Guglielmone AA. Ticks (Acari: Ixodoidea: Argasidae, Ixodidae) of Chile. Exp Appl Acarol. 2005;35:147–63. DOIPubMedGoogle Scholar
- Sanogo YO, Davoust B, Inokuma H, Camicas JL, Parola P, Brouqui P. First evidence of Anaplasma platys in Rhipicephalus sanguineus (Acari: Ixodida) collected from dogs in Africa. Onderstepoort J Vet Res. 2003;70:205–12.PubMedGoogle Scholar
- López J, Castillo A, Muñoz M, Hildebrand S. Hallazgo de Ehrlichia canis en Chile, informe preliminar. Archivos de Medicina Veterinaria. 1999;31:211–4. DOIGoogle Scholar
- López J, Rivera M, Concha JC, Gatica S, Loeffeholz M, Barriga O. Serologic evidence for human ehrlichiosis in Chile. Rev Med Chil. 2003;131:67–70.PubMedGoogle Scholar
- Harvey JW, Simpson CF, Gaskin JM. Cyclic thrombocytopenia induced by a Rickettsia-like agent in dogs. J Infect Dis. 1978;137:182–8.PubMedGoogle Scholar
- Aguirre E, Tesouro MA, Ruiz L, Amusategui I, Sainz A. Genetic characterization of Anaplasma (Ehrlichia) platys in dogs in Spain. J Vet Med B Infect Dis Vet Public Health. 2006;53:197–200. DOIPubMedGoogle Scholar
- de la Fuente J, Torina A, Naranjo V, Nicosia S, Alongi A, La Mantia F, Molecular characterization of Anaplasma platys strains from dogs in Sicily, Italy. BMC Vet Res. 2006;2:24. DOIPubMedGoogle Scholar
- Huang H, Unver A, Pérez MJ, Orellana NG, Rikihisa Y. Prevalence and molecular analysis of Anaplasma platys in dogs in Lara. Venezuela. Braz J Microbiol. 2005;36:211–6. DOIGoogle Scholar
- Arraga-Alvarado C, Palmar M, Parra O, Salas P. Fine structural characterisation of a Rickettsia-like organism in human platelets from patients with symptoms of ehrlichiosis. J Med Microbiol. 1999;48:991–7. DOIPubMedGoogle Scholar
- Arraga-Alvarado C, Montero-Ojeda M, Bernardoni A, Anderson BE, Parra O. Human ehrlichiosis: report of the 1st case in Venezuela. Invest Clin. 1996;37:35–49.PubMedGoogle Scholar
Page created: July 01, 2010
Page updated: July 01, 2010
Page reviewed: July 01, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.