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Volume 13, Number 9—September 2007
Dispatch

Anaplasma platys in Dogs, Chile

Katia Abarca*Comments to Author , Javier López†‡, Cecilia Perret*, Javier Guerrero†, Paula Godoy*, Ana Veloz*, Fernando Valiente-Echeverría*, Ursula León*, Constanza Gutjahr†, and Teresa Azócar*
Author affiliations: *Pontificia Universidad Católica de Chile, Santiago, Chile; †Faculty of Veterinary Medicine, Universidad Santo Tomás, Santiago, Chile; ‡Alcántara Veterinary Clinic, Santiago, Chile;

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Figure 1

A) Anaplasma platys nested PCR products of 30 blood samples from dogs in Chile. Positive samples from dogs 7, 12, 17, 19, 23, and 25 are indicated by a 150-bp band. –, PCR-negative control; dog 13, negative control; M, 50-bp DNA ladder. Value on the right is in basepairs. B) Second-round A. platys groESL nested PCR products of dog DNA samples with 3 sets of primers. Group A, SQ5F/SQ4R (790 bp); group B, SQ3F/SQ4R (1,170 bp); group C, SQ3F/SQ6R (360 bp). M, GeneRuler 1-kb DNA ladder (Fermentas, Hanover, MD, USA); Dog 13, negative control; –, PCR-negative control. Values on the left are in basepairs.

Figure 1. A) Anaplasma platys nested PCR products of 30 blood samples from dogs in Chile. Positive samples from dogs 7, 12, 17, 19, 23, and 25 are indicated by a 150-bp band. –, PCR-negative control; dog 13, negative control; M, 50-bp DNA ladder. Value on the right is in basepairs. B) Second-round A. platys groESL nested PCR products of dog DNA samples with 3 sets of primers. Group A, SQ5F/SQ4R (790 bp); group B, SQ3F/SQ4R (1,170 bp); group C, SQ3F/SQ6R (360 bp). M, GeneRuler 1-kb DNA ladder (Fermentas, Hanover, MD, USA); Dog 13, negative control; –, PCR-negative control. Values on the left are in basepairs.

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