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Volume 14, Number 11—November 2008
Dispatch

Use of Malaria Rapid Diagnostic Test to Identify Plasmodium knowlesi Infection

Thomas F. McCutchanComments to Author , Robert C. Piper, and Michael T. Makler
Author affiliations: National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA (T.F. McCutchan); University of Iowa, Iowa City, Iowa, USA (R.C. Piper); Flow Incorporated, Portland, Oregon, USA (M.T. Makler)

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Figure 1

Binding specificity of different anti–Plasmodium lactate dehydrogenase (pLDH) antibodies. A) Shown are the reactivities of the indicated monoclonal antibodies (MAbs) to the LDH from 7 Plasmodium spp. Reactivity was determined by using an immunocapture assay as previously described (9). B) Example of an immunodipstick assay that detects P. knowlesi. An immunochromatographic strip assay containing the indicated antibodies was allowed to wick lysed blood infected with P. vivax, P. falciparum, P. kn

Figure 1. Binding specificity of different anti–Plasmodium lactate dehydrogenase (pLDH) antibodies. A) Shown are the reactivities of the indicated monoclonal antibodies (MAbs) to the LDH from 7 Plasmodium spp. Reactivity was determined by using an immunocapture assay as previously described (9). B) Example of an immunodipstick assay that detects P. knowlesi. An immunochromatographic strip assay containing the indicated antibodies was allowed to wick lysed blood infected with P. vivax, P. falciparum, P. knowlesi, P. ovale, or P. malariae. Blood was wicked in the presence of colloidal gold conjugated to antibody 6C9, which binds all pLDH isoforms. P. vivax LDH is immobilized only by 11D9 and 13H11, and P. falciparum LDH was only immobilized by 17E4. P. knowlesi LDH was immobilized by 11D9 and 13H11 antibodies and also by 17E4. C) An immunochromatographic strip assay containing the indicated antibodies was allowed to wick lysed blood infected with P. vivax, P. cynomolgi, P. inui, and P. knowlesi. Blood was wicked in the presence of colloidal gold conjugated to antibody 6C9, which binds all pLDH isoforms. Both P. cynomolgi and P. inui show the same epitope profile as P. vivax.

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