Volume 14, Number 2—February 2008
Research
Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum
, and the Australian Scedosporium (AUSCEDO) Study Group
Table 1
Primer and adaptor oligonucleotide sequences used in the study
| Primer or adaptor oligonucelotide | Sequence (5′→3′) |
|---|---|
| rDNA primers | |
| SR6R | AAGTARAAGTCGTAACAAGG |
| LR1 | GGTTGGTTTCTTTTCCT |
| M13 fingerprinting primer | |
| Phage M13 | GAGGGTGGCGGTTCT |
| EcoRI adapters | |
| EA1 | CTCGTAGACTGCGTACC |
| EA2 | CATCTGACGCATGGTTAA |
| Msel adapters | |
| MA1 | GACGATGAGTCCTGAG |
| MA2 | TACTCAGGACTCAT |
| Preselective primers | |
| EcoRI-T | GACTGCGTACCAATTCT |
| EcoRI-G | GACTGCGTACCAATTCG |
| Msel-C | GATGAGTCCTGAGTAAC |
| Msel-G | GATGAGTCCTGAGTAAG |
| Selective primers | |
| EcoRI-TG | 6 FAM-GACTGCGTACCAATTCTG |
| EcoRI-GT | 6 FAM-GACTGCGTACCAATTCGT |
| Msel-CA | GATGAGTCCTGAGTAACA |
| Msel-GT | GATGAGTCCTGAGTAAGT |
1These authors contributed equally to experimental work and data analysis.
2Current affiilation: Lille Pasteur Institute, Lille, France
3Members of the Australian Scedosporium Study Group of the Australasian Society for Infectious Diseases: Australian Capital Territory: Peter Collignon; The Canberra Hospital. New South Wales: Richard Benn (Royal Prince Alfred Hospital); Ian Chambers (Douglass Hanly Moir Pathology); Sharon Chen (Westmead Hospital); Nelson Dennis (Wollongong Hospital); Deo DeWit (Gosford Hospital); John Ferguson (John Hunter Hospital); Iain Gosbell (Liverpool Hospital); Thomas Gottlieb (Concord Hospital); Catriona Halliday (Westmead Hospital); Juliette Holland (Mayne Laverty Pathology); Alison Kesson (New Children’s Hospital, Westmead); Richard Lawrence (St. George Hospital); Deborah Marriott (St. Vincent’s Hospital, Sydney); Wieland Meyer (Westmead Hospital); Peter Newton (Wollongong Hospital); Quoc Nguyen (St. Vincent’s Hospital, Sydney); Pamela Palasanthrian (Sydney Children’s Hospital); Robert Pickles (infectious diseases physician, Taree), Robert Pritchard (Royal North Shore Hospital); Tania Sorrell (Westmead Hospital); Lex Tierney (John Hunter Hospital); Voula Tomasotos (Liverpool Hospital); Robert Vaz (Orange Base Hospital); Kerry Weeks (Royal North Shore Hospital). Queensland: Anthony Allworth (Royal Brisbane Hospital); Christopher Coulter (The Prince Charles Hospital); Joan Faoagali (Royal Brisbane Hospital); Barbara Johnson (Princess Alexandra Hospital), David Looke (Princess Alexandra Hospital), Joseph McCormack (The Mater Adult Hospital); Graeme Nimmo (Princess Alexandra Hospital); Gabrielle O’Kane (The Prince Charles Hospital); E. Geoffrey Playford (Princess Alexandra Hospital); Jennifer Robson (Sullivan and Nicolaides Pathology). South Australia: David Ellis (Women’s and Children’s Hospital); Rosemary Handke (Women’s and Children’s Hospital); Karen Rowlands (Royal Adelaide Hospital); David Shaw(Royal Adelaide Hospital). Tasmania: Louise Cooley (Royal Hobart Hospital); Erica Cox (Launceston General Hospital); Alistair McGregor (Royal Hobart Hospital). Victoria: Clare Franklin (Alfred Hospital); Cathy Joseph (St Vincent’s Hospital, Melbourne), Tony Korman (Monash Medical Centre), Orla Morrissey (Alfred Hospital), Monica Slavin (Peter MacCallum Cancer Centre), Denis Spelman (Alfred Hospital); Bryan Speed (Austin and Repatriation Hospitals); Harsha Sheorey (St. Vincent’s Hospital, Melbourne). Western Australia: Peter Boan (Royal Perth Hospital); John Dyer (Fremantle Hospital); Christopher Heath (Royal Perth Hospital); Dianne Gardam (Royal Perth Hospital); Duncan McLennan (Fremantle Hospital); Ronan Murray (Royal Perth Hospital); Todd Pryce (Royal Perth Hospital).