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Volume 14, Number 6—June 2008

Influenza A Virus (H3N8) in Dogs with Respiratory Disease, Florida

Sunchai Payungporn*, P. Cynda Crawford†, Theodore S. Kouo*, Li-mei Chen*, Justine Pompey*, William L. Castleman†, Edward J. Dubovi‡, Jacqueline M. Katz*, and Ruben O. Donis*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †University of Florida, Gainesville, Florida, USA; ‡Cornell University, Ithaca, New York, USA;

Main Article

Table 1

Primers and probes for identification of canine influenza virus (H3N8) in tissues by quantitative real-time reverse transcription–PCR*

Primer Target Sequence Application
Ca-H3-F387 H3 (nt 387–406) 5′-tatgcatcgctccgatccat-3′ Forward primer for H3
Ca-H3-R487 H3 (nt 487–467) 5′-gctccacttcttccgttttga-3′ Reverse primer for H3
Ca-H3-P430 H3 (nt 430–459) FAM-aattcacagcagagggattcacatggacag-BHQ1 TaqMan probe
FluA-M-F151 M (nt 151–174) 5′-catggartggctaaagacaagacc-3′ Forward primer for M
FluA-M-R276 M (nt 276–253) 5′-agggcattttggacaaakcgtcta-3′ Reverse primer for M
FluA-M-P218 M (nt 218–235) FAM-acgcTcaccgTgcccAgt-BHQ1 TaqMan probe

*Conserved regions of the matrix (M) and hemagglutinin 3 (H3) genes of canine influenza virus were selected. The underlined r represents a mixture of a and g nucleotides at this position in the oligonucleotide; the underlined k represents mixtures of g or t; uppercase letters in the sequence for the M probe represent locked nucleic acids.

Main Article

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Page updated: July 09, 2010
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