Volume 14, Number 6—June 2008
Influenza A Virus (H3N8) in Dogs with Respiratory Disease, Florida
|Dog||Location||Duration of clinical disease, d||Real-time reverse transcription–PCR
|18S rRNA (Ct)||M (Ct)||M:rRNA||H3 (Ct)||H3:rRNA||Virus isolation|
|1078||Shelter||3||26.82||30.37||1.13||29.71||1.11||MDCK cells 3rd passage|
|374||Veterinary clinic||3||22.89||24.05||1.05||22.65||0.99||Egg 2nd passage|
|Normal dog lung||20.78||>40||>1.92||>40||>1.92||NA|
*Ct, cycle threshold; NVD, no virus detected; NA, not applicable; M:rRNA and H3:rRNA ratios were calculated by dividing the matrix (M) or hemagglutinin 3 (H3) Ct by the 18S rRNA Ct.
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