Volume 14, Number 6—June 2008
Research
Influenza A Virus (H3N8) in Dogs with Respiratory Disease, Florida
Sunchai Payungporn*, P. Cynda Crawford†, Theodore S. Kouo*, Li-mei Chen*, Justine Pompey*, William L. Castleman†, Edward J. Dubovi‡, Jacqueline M. Katz*, and Ruben O. Donis*
Table 2
Quantitative real-time reverse transcription–PCR and virus isolation from tissue specimens*
| Dog | Location | Duration of clinical disease, d | Real-time reverse transcription–PCR |
|||||
|---|---|---|---|---|---|---|---|---|
| 18S rRNA (Ct) | M (Ct) | M:rRNA | H3 (Ct) | H3:rRNA | Virus isolation | |||
| 1079 | Shelter | 2 | 26.18 | 29.81 | 1.14 | 28.84 | 1.10 | NVD |
| 1078 | Shelter | 3 | 26.82 | 30.37 | 1.13 | 29.71 | 1.11 | MDCK cells 3rd passage |
| 1080 | Shelter | 6 | 24.17 | 38.87 | 1.61 | 38.23 | 1.58 | NVD |
| 319 | Shelter | 6 | 24.94 | 33.87 | 1.36 | 32.23 | 1.29 | NVD |
| 318 | Shelter | 9 | 23.54 | 33.89 | 1.44 | 32.97 | 1.40 | NVD |
| 320 | Shelter | 10 | 23.91 | 39.44 | 1.65 | 37.09 | 1.55 | NVD |
| 374 | Veterinary clinic | 3 | 22.89 | 24.05 | 1.05 | 22.65 | 0.99 | Egg 2nd passage |
| A/canine/Florida/242/2003 | 29.37 | 28.15 | 0.96 | 27.36 | 0.93 | NA | ||
| Normal dog lung | 20.78 | >40 | >1.92 | >40 | >1.92 | NA | ||
*Ct, cycle threshold; NVD, no virus detected; NA, not applicable; M:rRNA and H3:rRNA ratios were calculated by dividing the matrix (M) or hemagglutinin 3 (H3) Ct by the 18S rRNA Ct.
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