Volume 15, Number 12—December 2009
Research
Landscape Epidemiology of Tularemia Outbreaks in Sweden
Table 1
Attributes of 7 novel typing markers for Francisella tularensis subsp. holarctica*
Marker category | Marker name | Genomic position† | Size, bp | Forward primer sequence (5′ →3′) | Reverse primer sequence (5′ → 3′) |
---|---|---|---|---|---|
INDEL | Ftind39 | 798173-4 | 11 | ATAATTACTATCAAATGCCCCAAC | CAAGATTTACCTCAAGAAATGGAT |
Ftind40 | 1502502-821 | 73 | ATATGATTGCTCCAGTATTTATTTC | TTGTAAGGTGATCGGAGTATTT | |
Ftind41 | 1494030-328 | 20 | CCAAGAGCAGAGCATAATTCTAA | GCCTGACRCAATGACATATTTAC | |
Ftind42 |
1849905-1850278 |
187 |
AGTAATAACGGTACGATCACAAAG |
GGCTTTAGCTTACCAACASAAC |
|
VNTR |
Ft-M26 |
1833026-37 |
6‡ |
AATACTCGCTTCTATCTTTCTGGT |
AATCTTTTGGAGAGGTTTTATTCA |
SNP | Ft-SNP1 | 927939 | 1 | ATCCCTGTTGGGATATCCTCGACTAA | ACCAAGGTAGATTTGCAGCTACA§ ACCAAGGTAGATTTGCAGCTACG§ |
Ft-SNP2 | 1044580 | 1 | atcagacttaggtgttagatcagagtt | tgaatactctacgcgataagata§ tgaatactctacgcgataagatg§ |
*INDEL, insertion/deletion; VNTR, variable number tandem repeat; SNP, single nucleotide polymorphism.
†GenBank accession no. AM233362.1 (complete genome of F. tularensis subsp. holarctica LVS).
‡There were two 6-bp repeats in the genome sequence of LVS.
§The 2 SNP states are in boldface, an adenosine nucleotide represent a derived state for both markers.