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Volume 16, Number 12—December 2010

Research

Reassortant Group A Rotavirus from Straw-colored Fruit Bat (Eidolon helvum)

Mathew D. Esona, Slavica Mijatovic-Rustempasic, Christina Conrardy, Suxiang Tong, Ivan V. Kuzmin, Bernard Agwanda, Robert F. Breiman, Krisztian Banyai, Michael Niezgoda, Charles E. Rupprecht, Jon R. Gentsch, and Michael D. BowenComments to Author 
Author affiliations: Author affilations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.D. Esona, S. Mijatovic-Rustempasic, C. Conrardy, S. Tong , I.V. Kuzmin, M. Niezgoda, C.E. Rupprecht, J.R. Gentsch, M.D. Bowen); National Museum of Kenya, Nairobi, Kenya (B. Agwanda); Centers for Disease Control and Prevention–Kenya, Nairobi (R.F. Breiman); Hungarian Academy of Sciences, Budapest, Hungary (K. Banyai)

Main Article

Table

Newly designed primers used for amplification and sequencing of bat rotavirus genes*

Primer Sequence, 5′ → 3′ Gene Nucleotide position, strand
VP1_Bat GCT TCG AAT GGA GAA TCG CG VP1 596–576, –
VP1_Bat CTC CTG GTG TGT ACC TAC C VP1 485–504, +
VP1_Bat CCT CGT GTG TAA ATA CGG ACA VP1 1130–1151, +
VP2_Bat CAG AGC AGG CTA AGA AGC AGA CTA VP2 393–370, –
VP2_Bat GCA GCA CCA ATT TGG GTT GAG VP2 810–830, +
VP2_Bat CTC AAC CCA AAT TGG TGC TG VP2 830–810, –
VP2_Bat CTG AAT CTG AGC TTC AGT TG VP2 1206–1187, –
VP2_Bat GAT GTA GCT AGA GTG CCA G VP2 2247–2265, +
VP2_Bat CTG GCA CTC TAG CTA CAT C VP2 2265–2247, –
VP2_Bat GGT GGC GAA TTA TGA TTG G VP2 2723–2741, +
VP6_Bat TCG GTC TAT GGA ATG TGA AAC CTG TTC VP6 380–354, –
VP7_Bat CAG ATG TCG TCG ATA ATG VP7 693–710, +
VP7_Bat TGG CGT ACG CAG TGT CCA TTG AC VP7 218–196, –
VP7_Bat CGA GTT GGC GTA CGC AGT G VP7 258–240, –
NSP2_Bat CAA CTT CCA CAT TGT AAA CGC NSP2 574–555, –
NSP2_Bat CTT TCG CGA ATA CAC TGG T NSP2 1087–1068, –
NSP2_Bat CT GAT AGA GTG TAT GCG AC NSP2 727–747, +
NSP3_Bat CCT CAC TCT CAT CTT TCG GGT CTT C NSP3 401–376, –
NSP3_Bat GGA GTT ACC GAG TGA AGC GAA GG NSP3 673–695, +
NSP5_Bat ACG CCA GCA TCT GCA TTT GTC NSP5 279–258, –

*VP, viral protein; NSP, nonstructural protein.

Main Article

Page created: August 29, 2011
Page updated: August 29, 2011
Page reviewed: August 29, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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