Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 16, Number 3—March 2010
Dispatch

Parvovirus 4–like Virus in Blood Products

Jozsef Szelei, Kaiyu Liu, Yi Li, Sandra Fernandes, and Peter TijssenComments to Author 
Author affiliations: Institut National de la Recherche Scientifique–Institut Armand-Frappier, Laval, Quebec, Canada (J. Szelei, K. Liu, Yi Li, S. Fernandes, P. Tijssen); Central People’s Republic of China Normal University, Wuhan, People’s Republic of China (Y. Li)

Main Article

Figure 1

Parallel PCR amplification of PARV4-like (A) and PPV (B) by using purified DNA from clotting FVIII preparations. The results of this PCR usually suggested a higher PARV4 load despite the higher efficiency of the PPV PCR (J. Szelei and P. Tijssen, unpub. data). This finding was confirmed with the quantitative MIMIC PCR method for PPV (11). Numbers indicate different lots of FVIII prepared in 1:1994A, 2:1994B, 3:1996A, 4:1996B, 5:1999, 6:2000A, 7:2000B, 8:2001A, 9:2001B, 10:2001C, 11:2001D, and 12

Figure 1. Parallel PCR amplification of PARV4-like (A) and PPV (B) by using purified DNA from clotting FVIII preparations. The results of this PCR usually suggested a higher PARV4 load despite the higher efficiency of the PPV PCR (J. Szelei and P. Tijssen, unpub. data). This finding was confirmed with the quantitative MIMIC PCR method for PPV (11). Numbers indicate different lots of FVIII prepared in 1:1994A, 2:1994B, 3:1996A, 4:1996B, 5:1999, 6:2000A, 7:2000B, 8:2001A, 9:2001B, 10:2001C, 11:2001D, and 12: DNA marker (1-kb ladder; Invitrogen, Carlsbad, CA USA). PARV4, parvovirus 4; PPV, porcine parvovirus; FVIII, factor VIII.

Main Article

References
  1. Jones  MS, Kapoor  A, Lukashov  VV, Simmonds  P, Hecht  F, Delwart  E. New DNA viruses identified in patients with acute viral infection syndrome. J Virol. 2005;79:8230–6. DOIPubMedGoogle Scholar
  2. Fryer  JF, Delwart  E, Bernardin  F, Tuke  PW, Lukashov  VV, Baylis  SA. Analysis of two human parvovirus PARV4 genotypes identified in human plasma for fractionation. J Gen Virol. 2007;88:2162–7. DOIPubMedGoogle Scholar
  3. Longhi  E, Bestetti  G, Acquaviva  V, Foschi  A, Piolini  R, Meroni  L, Human parvovirus 4 in the bone marrow of Italian patients with AIDS. AIDS. 2007;21:1481–3. DOIPubMedGoogle Scholar
  4. Sharp  CP, Lail  A, Donfield  S, Simmons  R, Leen  C, Klenerman  P, High frequencies of exposure to the novel human parvovirus PARV4 in hemophiliacs and injection drug users, as detected by a serological assay for PARV4 antibodies. J Infect Dis. 2009;200:1119–25. DOIPubMedGoogle Scholar
  5. Simmonds  P, Manning  A, Kenneil  R, Carnie  FW, Bell  JE. Parenteral transmission of the novel human parvovirus PARV4. Emerg Infect Dis. 2007;13:1386–8.PubMedGoogle Scholar
  6. Schneider  B, Fryer  JF, Oldenburg  J, Brackmann  HH, Baylis  SA, Eis-Hübinger  AM. Frequency of contamination of coagulation factor concentrates with novel human parvovirus PARV4. Haemophilia. 2008;14:978–86. DOIPubMedGoogle Scholar
  7. Kleinman  SH, Glynn  SA, Lee  TH, Tobler  LH, Schlumpf  KS, Todd  DS, A linked donor-recipient study to evaluate parvovirus B19 transmission by blood component transfusion. Blood. 2009;114:3677–83. DOIPubMedGoogle Scholar
  8. Manning  A, Willey  SJ, Bell  JE, Simmonds  P. Comparison of tissue distribution, persistence, and molecular epidemiology of parvovirus B19 and novel human parvoviruses PARV4 and human bocavirus. J Infect Dis. 2007;195:1345–52. DOIPubMedGoogle Scholar
  9. Simmonds  P, Douglas  J, Bestetti  G, Longhi  E, Antinori  S, Parravicini  C, A third genotype of the human parvovirus PARV4 in sub-Saharan Africa. J Gen Virol. 2008;89:2299–302. DOIPubMedGoogle Scholar
  10. Lau  SK, Woo  PC, Tse  H, Fu  CT, Au  WK, Chen  XC, Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4. J Gen Virol. 2008;89:1840–8. DOIPubMedGoogle Scholar
  11. Zádori  Z, Szelei  J, Lacoste  MC, Li  Y, Gariépy  S, Raymond  P, A viral phospholipase A2 is required for parvovirus infectivity. Dev Cell. 2001;1:291–302. DOIPubMedGoogle Scholar
  12. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9. DOIPubMedGoogle Scholar
  13. Zádori  Z, Szelei  J, Tijssen  P. SAT: a late NS protein of porcine parvovirus. J Virol. 2005;79:13129–38. DOIPubMedGoogle Scholar

Main Article

Page created: December 14, 2010
Page updated: December 14, 2010
Page reviewed: December 14, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external