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Volume 16, Number 6—June 2010
Research

Evolution of Northeastern and Midwestern Borrelia burgdorferi, United States

Dustin BrissonComments to Author , Mary F. Vandermause, Jennifer K. Meece, Kurt D. Reed, and Daniel E. Dykhuizen
Author affiliations: University of Pennsylvania, Philadelphia, Pennsylvania, USA (D. Brisson); Marshfield Clinic Research Foundation, Marshfield, Wisconsin, USA (M.F. Vandermause, J.K. Meece); Northwestern University/Feinberg School of Medicine, Chicago, Illinois, USA (K.D. Reed); Stony Brook University, Stony Brook, New York, USA (D.E. Dykhuizen)

Main Article

Figure

Phylogeny of Borrelia burgdorferi isolates in the northeastern and midwestern United States based on intergenic spacer (IGS) sequence. operational taxanomic unit names beginning with IGS were isolated in the northeastern United States (10); all other isolates are from patients in the Midwest. The letter after period designates the outer surface protein C (ospC) major allele of the isolate. Colored isolate names highlight isolates with the same ospC major group that cluster in different clades, w

Figure. Phylogeny of Borrelia burgdorferi isolates in the northeastern and midwestern United States based on intergenic spacer (IGS) sequence. operational taxanomic unit names beginning with IGS were isolated in the northeastern United States (10); all other isolates are from patients in the Midwest. The letter after period designates the outer surface protein C (ospC) major allele of the isolate. Colored isolate names highlight isolates with the same ospC major group that cluster in different clades, which suggests horizontal gene transfer. The ospC of several strains is not linked to the IGS ribosomal spacer type (RST) to which it is commonly linked in the Northeast (10,34). AB indicates differences between the ospAB tree and the IGS tree. This tree is midpoint rooted. Scale bar indicates number of substitutions per site.

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References
  1. Bacon  RM, Kugeler  KJ, Mead  PS. Surveillance for Lyme disease—United States, 1992–2006. MMWR Surveill Summ. 2008;57:19.PubMed
  2. Burgdorfer  W, Barbour  AG, Hayes  SF, Benach  JL, Grunwaldt  E, Davis  JP. Lyme disease—a tick-borne spirochetosis. Science. 1982;216:13179. DOIPubMed
  3. Gatewood  AG, Liebman  KA, Vourc’h  G, Bunikis  J, Hamer  SA, Cortinas  R, Climate and tick seasonality are predictors of Borrelia burgdorferi genotype distribution. Appl Environ Microbiol. 2009;75:247683. DOIPubMed
  4. Brisson  D, Dykhuizen  DE. ospC diversity in Borrelia burgdorferi: different hosts are different niches. Genetics. 2004;168:71322. DOIPubMed
  5. Caporale  DA, Johnson  CM, Millard  BJ. Presence of Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) in southern Kettle Moraine State Forest, Wisconsin, and characterization of strain W97F51. J Med Entomol. 2005;42:45772. DOIPubMed
  6. Diuk-Wasser  MA, Gatewood  AG, Cortinas  MR, Yaremych-Hamer  S, Tsao  J, Kitron  U, Spatiotemporal patterns of host-seeking Ixodes scapularis nymphs (Acari: Ixodidae) in the United States. J Med Entomol. 2006;43:16676. DOIPubMed
  7. Qiu  WG, Dykhuizen  DE, Acosta  MS, Luft  BJ. Geographic uniformity of the Lyme disease spirochete (Borrelia burgdorferi) and its shared history with tick vector (Ixodes scapularis) in the northeastern United States. Genetics. 2002;160:83349.PubMed
  8. Humphrey  PT, Caporale  DA, Brisson  D. Uncoordinated biogeography of the Lyme disease pathogen, Borrelia burgdorferi, and its tick vector, Ixodes scapularis. Evolution. 2010. In press. DOIPubMed
  9. Qiu  WG, Schutzer  SE, Bruno  JF, Attie  O, Xu  Y, Dunn  JJ, Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing. Proc Natl Acad Sci U S A. 2004;101:141505. DOIPubMed
  10. Bunikis  J, Tsao  J, Berglund  J, Fish  D, Barbour  AG. Sequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe. Microbiology. 2004;150:174155. DOIPubMed
  11. Dykhuizen  DE, Polin  DS, Dunn  JJ, Wilske  B, Preac-Mursic  V, Dattwyler  RJ, Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development. Proc Natl Acad Sci U S A. 1993;90:101637. DOIPubMed
  12. Attie  O, Bruno  JF, Xu  Y, Qiu  D, Luft  BJ, Qiu  WG. Co-evolution of the outer surface protein C gene (ospC) and intraspecific lineages of Borrelia burgdorferi sensu stricto in the northeastern United States. Infect Genet Evol. 2007;7:112. DOIPubMed
  13. Dykhuizen  DE, Baranton  G. The implications of a low rate of horizontal transfer in Borrelia. Trends Microbiol. 2001;9:34450. DOIPubMed
  14. Guttman  DS, Wang  PW, Wang  IN, Bosler  EM, Luft  BJ, Dykhuizen  DE. Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis. J Clin Microbiol. 1996;34:6526.PubMed
  15. Casjens  S, Palmer  N, van Vugt  R, Huang  WM, Stevenson  B, Rosa  P, A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol Microbiol. 2000;35:490516. DOIPubMed
  16. Stevenson  B, Miller  JC. Intra- and interbacterial genetic exchange of Lyme disease spirochete erp genes generates sequence identity amidst diversity. J Mol Evol. 2003;57:30924. DOIPubMed
  17. Zhang  J-R, Norris  SJ. Genetic variation of the Borrelia burgdorferi gene vlsE involves cassette-specific, segmental gene conversion. Infect Immun. 1998;66:3698704.PubMed
  18. Shimodaira  H, Hasegawa  M. Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Mol Biol Evol. 1999;16:11146.
  19. Wormser  GP, Liveris  D, Nowakowski  J, Nadelman  RB, Cavaliere  LF, McKenna  D, Association of specific subtypes of Borrelia burgdorferi with hematogenous dissemination in early Lyme disease. J Infect Dis. 1999;180:7205. DOIPubMed
  20. Caporale  DA, Kocher  TD. Sequence variation in the outer-surface-protein genes of Borrelia burgdorferi. Mol Biol Evol. 1994;11:5164.PubMed
  21. Thompson  JD, Higgins  DG, Gibson  TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:467380. DOIPubMed
  22. Rozas  J, Rozas  R. DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics. 1999;15:1745. DOIPubMed
  23. Jolley  KA, Feil  EJ, Chan  MS, Maiden  MC. Sequence type analysis and recombinational tests (START). Bioinformatics. 2001;17:12301. DOIPubMed
  24. Sawyer  S. Statistical tests for detecting gene conversion. Mol Biol Evol. 1989;6:52638.PubMed
  25. Smith  JM. Analyzing the mosaic structure of genes. J Mol Evol. 1992;34:1269. DOIPubMed
  26. Smith  JM, Smith  NH, O’Rourke  M, Spratt  BG. How clonal are bacteria? Proc Natl Acad Sci U S A. 1993;90:43848. DOIPubMed
  27. Seinost  G, Dykhuizen  DE, Dattwyler  RJ, Golde  WT, Dunn  JJ, Wang  IN, Four clones of Borrelia burgdorferi sensu stricto cause invasive infection in humans. Infect Immun. 1999;67:351824.PubMed
  28. Dykhuizen  DE, Brisson  D, Sandigursky  S, Wormser  GP, Nowakowski  J, Nadelman  RB, The propensity of different Borrelia burgdorferi sensu stricto genotypes to cause disseminated infections in humans. Am J Trop Med Hyg. 2008;78:80610.PubMed
  29. Posada  D, Crandall  KA. MODELTEST: testing the model of DNA substitution. Bioinformatics. 1998;14:8178. DOIPubMed
  30. Wormser  GP, Brisson  D, Liveris  D, Hanincová  K, Sandigursky  S, Nowakowski  J, Borrelia burgdorferi genotype predicts the capacity for hematogenous dissemination during early Lyme disease. J Infect Dis. 2008;198:135864. DOIPubMed
  31. Liveris  D, Wormser  GP, Nowakowski  J, Nadelman  R, Bittker  S, Cooper  D, Molecular typing of Borrelia burgdorferi from Lyme disease patients by PCR-restriction fragment length polymorphism analysis. J Clin Microbiol. 1996;34:13069.PubMed
  32. Livey  I, Gibbs  CP, Schuster  R, Dorner  F. Evidence for lateral transfer and recombination in OspC variation in Lyme disease Borrelia. Mol Microbiol. 1995;18:25769. DOIPubMed
  33. Ragan  MA. Detection of lateral gene transfer among microbial genomes. Curr Opin Genet Dev. 2001;11:6206. DOIPubMed
  34. Wang  G, Ojaimi  C, Wu  H, Saksenberg  V, Iyer  R, Liveris  D, Disease severity in a murine model of Lyme borreliosis is associated with the genotype of the infecting Borrelia burgdorferi sensu stricto strain. J Infect Dis. 2002;186:78291. DOIPubMed
  35. Cromley  EK, Cartter  ML, Mrozinski  RD, Ertel  SH. Residential setting as a risk factor for Lyme disease in a hyperendemic region. Am J Epidemiol. 1998;147:4727.PubMed
  36. Brisson  D, Dykhuizen  DE. A modest model explains the distribution and abundance of Borrelia burgdorferi strains. Am J Trop Med Hyg. 2006;74:61522.PubMed
  37. Brisson  D, Dykhuizen  DE, Ostfeld  RS. Conspicuous impacts of inconspicuous hosts on the Lyme disease epidemic. Proc Biol Sci. 2008;275:22735. DOIPubMed

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