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Volume 17, Number 3—March 2011
Research
Amplification of Emerging Viruses in a Bat Colony
Jan Felix Drexler1, Victor Max Corman1, Tom Wegner, Adriana Fumie Tateno, Rodrigo Melim Zerbinati, Florian Gloza-Rausch, Antje Seebens, Marcel A. Müller, and Sung Sup Park
Table 1
Real-time reverse transcription–PCR oligonucleotides used for RNA virus testing, Germany, 2008–2010*
| Virus targeted | Oligonucleotide ID | Sequence, 5′ → 3′ | Orientation |
|---|---|---|---|
| Coronavirus |
CoV-F | CGTCTGGTGATGCTACTACTGCTT | + |
| CoV-P | FAM-TGCAAATTCCGTCTTTAAT-MGBNFQ | Probe | |
| CoV-R |
CATTGGCACTAACAGCCTGAAA |
– |
|
| Astrovirus |
AstVa-F | GCTTGATCCWGTCTATCATACTGATG | + |
| AstVa-P | FAM-CTTTTGAGTTTGCGTATGTTCA-MGBNFQ | Probe | |
| AstVa-R | CACATTTTTTCCATTCTTCTTCAAG | – | |
| AstVb-F | TATGTACTACTGCCTTCTGGTGAAATC | + | |
| AstVb-P | YAK-CCCACCAAACTCGCGGGAATCCT-BBQ1 | Probe | |
| AstVb-R |
TTATCCATCGTTGTGCTCACTTG |
– |
|
| Adenovirus | AdV-F | GCGGTTGCAGCTAAGATTTGT | + |
| AdV-P | FAM-CCCGTGGACAAAGAAGACACCCAGTATG-BBQ1 | Probe | |
| AdV-R | CCAGCTGGAAGCGTGTTTTAT | – |
*ID, identification; CoV, coronavirus; AstV, atrovirus; AdV, adenovirus; FAM, 6-carboxyfluorescein; MGB, minor groove binder; NFQ, nonfluorescent quencher; YAK, Yakima yellow; BBQ, black berry quencher; +, positive; –, negative.
1These authors contributed equally to this article.
- Page created: July 25, 2011
- Page last updated: July 25, 2011
- Page last reviewed: July 25, 2011
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