Volume 17, Number 5—May 2011
Research
Experimental Oral Transmission of Atypical Scrapie to Sheep
Marion M. Simmons
, S. Jo Moore1, Timm Konold, Lisa Thurston, Linda A. Terry, Leigh Thorne, Richard Lockey, Chris Vickery, Stephen A.C. Hawkins, Melanie J. Chaplin, and John Spiropoulos
, S. Jo Moore1, Timm Konold, Lisa Thurston, Linda A. Terry, Leigh Thorne, Richard Lockey, Chris Vickery, Stephen A.C. Hawkins, Melanie J. Chaplin, and John Spiropoulos
Appendix
Table. Immunohistochemical/ELISA results for sheep infected with scrapie for each tissue*
| Genotype |
ARR/ARR |
AHQ/AHQ |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Months postinoculation |
12 |
24 |
12 |
24 |
|||||||||||
| Donor inoculum |
ARRa |
ARRa |
ARRa |
ARRa |
ARRa |
ARRb |
AHQ |
AHQ |
AHQ |
AHQ |
AHQ |
AHQ |
|||
| Animal no. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |||
| Clinical status | Norm | Norm | Inc | Norm | Norm | Inc | Norm | Norm | Norm | Inc | Norm | Suspect | |||
| Obex | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Cerebellum† | −/−† | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/−‡ | +/+‡ | |||
| Rostral medulla | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Caudal midbrain | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Rostral midbrain | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Thalamus | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/−‡§ | +/+ | |||
| Occipital cortex | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Parietal cortex | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Frontal cortex | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/+‡ | +/+ | |||
| Spinal cord C5 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Spinal cord T1 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Spinal cord T6 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Spinal cord T12 | −/− | −/− | –/ITT | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |||
| Spinal cord L5–6 | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Palatine tonsil | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | –/ITT | |||
| Spleen† | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |||
| Mesenteric lymph node | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |||
| Distal ileum† | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/−‡ | −/−‡ | −/− | −/− | −/−‡ | |||
| RAMALT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | |||
| Coeliaco-mesenteric plexus | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | –/NT | ITT/NT | –/NT | –/NT | –/NT | |||
| Extraocular muscles | –/ITT | –/ITT | –/ITT | −/− | –/ITT | −/− | –/ITT | −/− | −/− | −/− | −/− | −/− | |||
| Medial retropharyngeal lymph node | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
| Submandibular lymph node | NT/ITT | NT/ITT | NT/ITT | NT/ITT | NT/– | NT/– | NT/– | NT/ITT | NT/ITT | NT/– | /ITT | −/− | |||
| Submandibular salivary gland | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
| Cervical thymus | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
| Prescapular lymph node | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
| Kidney | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
| Liver | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | NT/– | −/− | −/− | |||
| Buffy coat | NT/– | NT/– | NT/– | NT/ITT | NT/ITT | NT/ITT | NT/– | NT/– | NT/– | NT/ITT | NT/ITT | NT/ITT | |||
*ARR/ARR, Prp genotype of 6 Poll Dorset lambs; AHQ/AHQ, PrP genotype of 6 Cheviot lambs; mpi, months post inoculation; Norm, normal; Inc, inconclusive; Suspect, suspected; NT, not tested; ITT, insufficient tissue to test; RAMALT, recto-anal mucosa–associated
lymphoid tissue; PrPSc or protease-resistant form present (+) or absent (–).
†Tissues being investigated by bioassay in Tg338 mice.
‡Tissues are positive in bioassay.
§High negative result in ELISA.
1Current affiliation: Murdoch University School of Veterinary and Biomedical Sciences, Murdoch, Western Australia, Australia.
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