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Volume 18, Number 1—January 2012
Dispatch

Molecular Evolution of Respiratory Syncytial Virus Fusion Gene, Canada, 2006–2010

Jesse Papenburg, Julie Carbonneau, Marie-Ève Hamelin, Sandra Isabel, Xavier Bouhy, Najwa Ohoumanne, Pierre Déry, Bosco A. Paes, Jacques Corbeil, Michel G. Bergeron, Gaston De Serres, and Guy BoivinComments to Author 
Author affiliations: McGill University Health Centre, Montréal, Québec, Canada (J. Papenburg); Centre de Recherche du Centre Hospitalier Universitaire de Québec, Québec City, Québec, Canada (J. Papenburg, J. Carbonneau, M.-È. Hamelin, S. Isabel, X. Bouhy, P. Déry, J. Corbeil, M.G. Bergeron, G. Boivin); Institut National de Santé Publique du Québec, Québec (N. Ohoumanne, G. De Serres); McMaster Children’s Hospital, Hamilton, Ontario, Canada (B.A. Paes)

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Figure

Figure. Phylogenetic analysis of 170 near–full-length unique respiratory syncitial virus fusion (RSV-F) gene sequences (nt 79–1602). Panels A and B are detailed phylograms of the RSV-A and RSV-B taxa analyzed, respectively. One bovine RSV-F sequence was added to the dataset (GenBank accession no. AF295543.1) as the outgroup (not shown) and used for rooting the phylograms. Topology was inferred by using the neighbor-joining method, and evolutionary distances were computed by using the maximum-composite likelihood method in MEGA5 software (www.megasoftware.net). The topologic accuracy of the tree was evaluated by using 1,000 bootstrap replicates. Only bootstrap values >50% are shown. Blue text and triangles represent RSV strains isolated in Canada and sequenced at the Centre de Recherche du Centre Hospitalier Universitaire de Québec; red branches indicates a sublineage of RSV-A with a N276S mutation; underlining indicates prototypical RSV A2 and RSV B1 strains. The clinical origin of strains from Canada (prospective study hospitalized patient [H] or clinic patient [C]; 2004–2005 palivizumab study patient [S]; retrospectively identified patient from the Montréal Children’s Hospital [MCH] or McMaster Children’s Hospital [MAC]) is indicated, followed by the year collected, the specimen identifier, and the result of RSV genogroup testing (RSV-A [A], RSV-B [B]) by multiplex PCR/DNA hybridization assay (14). Specimens with a nonsilent mutation at codon 272 have the amino acid substitution identified in brackets. When a taxon represents >1 identical sequences, the number of patients that it represents and the number of palivizumab recipients (PZB) among these patients are noted in parentheses. SA, South Africa; CHN, People’s Republic of China; UK, United Kingdom, JPN, Japan; AUS, Australia; USA, United States; CDC, US Centers for Disease Control and Prevention; NY, New York; SL, St. Louis; WV, West Virginia; MN, Minnesota; MD, Maryland; WA, Washington; SEL, Seoul, South Korea. Scale bars represent substitutions per basepair per the indicated horizontal distance.

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References
  1. Nair  H, Nokes  DJ, Gessner  BD, Dherani  M, Madhi  SA, Singleton  RJ, Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. Lancet. 2010;375:1545–55. DOIPubMedGoogle Scholar
  2. Welliver  RC. Review of epidemiology and clinical risk factors for severe respiratory syncytial virus (RSV) infection. J Pediatr. 2003;143:S112–7. DOIPubMedGoogle Scholar
  3. Collins  PL, Crowe  JE. Respiratory syncytial virus and metapneumovirus. In: Knipe DM, Howley PM, editors. Field’s virology.,5th ed. New York: Lippincott Williams and Wilkins; 2007. p. 1601–46.
  4. Crowe  JE, Firestone  CY, Crim  R, Beeler  JA, Coelingh  KL, Barbas  CF, Monoclonal antibody-resistant mutants selected with a respiratory syncytial virus–neutralizing human antibody fab fragment (Fab 19) define a unique epitope on the fusion (F) glycoprotein. Virology. 1998;252:373–5. DOIPubMedGoogle Scholar
  5. Huang  K, Incognito  L, Cheng  X, Ulbrandt  ND, Wu  H. Respiratory syncytial virus–neutralizing monoclonal antibodies motavizumab and palivizumab inhibit fusion. J Virol. 2010;84:8132–40. DOIPubMedGoogle Scholar
  6. Committee on Infectious Diseases. From the American Academy of Pediatrics: policy statements–modified recommendations for use of palivizumab for prevention of respiratory syncytial virus infections. Pediatrics. 2009;124:1694–701. DOIPubMedGoogle Scholar
  7. Zhao  X, Chen  FP, Megaw  AG, Sullender  WM. Variable resistance to palivizumab in cotton rats by respiratory syncytial virus mutants. J Infect Dis. 2004;190:1941–6. DOIPubMedGoogle Scholar
  8. Adams  O, Bonzel  L, Kovacevic  A, Mayatepek  E, Hoehn  T, Vogel  M. Palivizumab-resistant human respiratory syncytial virus infection in infancy. Clin Infect Dis. 2010;51:185–8. DOIPubMedGoogle Scholar
  9. Zhu  Q, McAuliffe  JM, Patel  NK, Palmer-Hill  FJ, Yang  CF, Liang  B, Analysis of respiratory syncytial virus preclinical and clinical variants resistant to neutralization by monoclonal antibodies palivizumab and/or motavizumab. J Infect Dis. 2011;203:674–82. DOIPubMedGoogle Scholar
  10. Kim  YK, Choi  EH, Lee  HJ. Genetic variability of the fusion protein and circulation patterns of genotypes of the respiratory syncytial virus. J Med Virol. 2007;79:820–8. DOIPubMedGoogle Scholar
  11. Zheng  H, Storch  GA, Zang  C, Peret  TC, Park  CS, Anderson  LJ. Genetic variability in envelope-associated protein genes of closely related group A strains of respiratory syncytial virus. Virus Res. 1999;59:89–99. DOIPubMedGoogle Scholar
  12. Gilca  R, De Serres  G, Tremblay  M, Vachon  ML, Leblanc  E, Bergeron  MG, Distribution and clinical impact of human respiratory syncytial virus genotypes in hospitalized children over 2 winter seasons. J Infect Dis. 2006;193:54–8. DOIPubMedGoogle Scholar
  13. Peret  TC, Hall  CB, Hammond  GW, Piedra  PA, Storch  GA, Sullender  WM, Circulation patterns of group A and B human respiratory syncytial virus genotypes in 5 communities in North America. J Infect Dis. 2000;181:1891–6. DOIPubMedGoogle Scholar
  14. Raymond  F, Carbonneau  J, Boucher  N, Robitaille  L, Boisvert  S, Wu  WK, Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children. J Clin Microbiol. 2009;47:743–50. DOIPubMedGoogle Scholar
  15. Boivin  G, Caouette  G, Frenette  L, Carbonneau  J, Ouakki  M, De Serres  G. Human respiratory syncytial virus and other viral infections in infants receiving palivizumab. J Clin Virol. 2008;42:52–7. DOIPubMedGoogle Scholar

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