Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 18, Number 4—April 2012
Research

Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157

Christian Jenke, Shana R. Leopold, Thomas Weniger, Jörg Rothgänger, Dag Harmsen, Helge Karch, and Alexander MellmannComments to Author 
Author affiliations: Institute for Hygiene and National Consulting Laboratory on Hemolytic Uremic Syndrome, Münster, Germany (C. Jenke, S.R. Leopold, H. Karch, A. Mellmann); University Hospital Münster Periodontology, Münster (T. Weniger, D. Harmsen); Ridom GmbH, Münster (J. Rothgänger)

Main Article

Figure

Minimum-spanning tree based on single-nucleotide polymorphism (SNP) genotypes illustrating the phylogeny of 50 enterohemorrhagic Escherichia coli O157:H7/H– and O55:H7 isolates and the intermediate position of strain LSU-61 during the evolution of O157. Each node represents a unique SNP genotype. The size of each node is proportional to the number of isolates per SNP genotype based on sequence analysis of 51,041 bp comprising 92 partial open reading frames. Numbers on lines between nodes represe

Figure. Minimum-spanning tree based on single-nucleotide polymorphism (SNP) genotypes illustrating the phylogeny of 50 enterohemorrhagic Escherichia coli O157:H7/H and O55:H7 isolates and the intermediate position of strain LSU-61 during the evolution of O157. Each node represents a unique SNP genotype. The size of each node is proportional to the number of isolates per SNP genotype based on sequence analysis of 51,041 bp comprising 92 partial open reading frames. Numbers on lines between nodes represent distances between the nodes, i.e., the number of SNPs. The node size is proportional to the number of strains sharing the same genotype. Strains are colored according to their classification into subgroups and clusters based on information from Saikh and Tarr (11) and Leopold et al. (12). Strain LSU-61 represents a potential intermediate interlinking all 3 subgroups. SF, sorbitol fermenting.

Main Article

References
  1. Holtz  LR, Neill  MA, Tarr  PI. Acute bloody diarrhea: a medical emergency for patients of all ages. Gastroenterology. 2009;136:188798. DOIPubMed
  2. Levine  MM. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis. 1987;155:37789. DOIPubMed
  3. Tarr  PI, Gordon  CA, Chandler  WL. Shiga toxin–producing Escherichia coli and haemolytic uraemic syndrome. Lancet. 2005;365:107386. DOIPubMed
  4. Karch  H, Tarr  PI, Bielaszewska  M. Enterohaemorrhagic Escherichia coli in human medicine. Int J Med Microbiol. 2005;295:40518. DOIPubMed
  5. Karch  H, Bielaszewska  M. Sorbitol-fermenting Shiga toxin–producing Escherichia coli O157:H– strains: epidemiology, phenotypic and molecular characteristics, and microbiological diagnosis. J Clin Microbiol. 2001;39:20439. DOIPubMed
  6. Karch  H, Mellmann  A, Bielaszewska  M. Epidemiology and pathogenesis of enterohaemorrhagic Escherichia coli. Berl Munch Tierarztl Wochenschr. 2009;122:41724.PubMed
  7. Pennington  H. Escherichia coli O157. Lancet. 2010;376:142835. DOIPubMed
  8. Werber  D, Bielaszewska  M, Frank  C, Stark  K, Karch  H. Watch out for the even eviler cousin—sorbitol-fermenting E coli O157. Lancet. 2011;377:2989. DOIPubMed
  9. Feng  P, Lampel  KA, Karch  H, Whittam  TS. Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. J Infect Dis. 1998;177:17503. DOIPubMed
  10. Feng  PCH, Monday  SR, Lacher  DW, Allison  L, Siitonen  A, Keys  C, Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model. Emerg Infect Dis. 2007;13:17016.PubMed
  11. Shaikh  N, Tarr  PI. Escherichia coli O157:H7 Shiga toxin–encoding bacteriophages: integrations, excisions, truncations, and evolutionary implications. J Bacteriol. 2003;185:3596605. DOIPubMed
  12. Leopold  SR, Magrini  V, Holt  NJ, Shaikh  N, Mardis  ER, Cagno  J, A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis. Proc Natl Acad Sci U S A. 2009;106:87138.PubMed
  13. Dunn  JR, Keen  JE, Moreland  D, Alex  T. Prevalence of Escherichia coli O157:H7 in white-tailed deer from Louisiana. J Wildl Dis. 2004;40:3615.PubMed
  14. Perna  NT, Plunkett  G III, Burland  V, Mau  B, Glasner  JD, Rose  DJ, Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature. 2001;409:52933. DOIPubMed
  15. Shaikh  N, Holt  NJ, Johnson  JR, Tarr  PI. Fim operon variation in the emergence of enterohemorrhagic Escherichia coli: an evolutionary and functional analysis. FEMS Microbiol Lett. 2007;273:5863. DOIPubMed
  16. Karch  H, Wiss  R, Gloning  H, Emmrich  P, Aleksic  S, Bockemühl  J. Hemolytic-uremic syndrome in infants due to verotoxin-producing Escherichia coli [in German]. Dtsch Med Wochenschr. 1990;115:48995. DOIPubMed
  17. Kulasekara  BR, Jacobs  M, Zhou  Y, Wu  Z, Sims  E, Saenphimmachak  C, Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhance virulence. Infect Immun. 2009;77:371321. DOIPubMed
  18. Hayashi  T, Makino  K, Ohnishi  M, Kurokawa  K, Ishii  K, Yokoyama  K, Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 2001;8:1122. DOIPubMed
  19. Jenke  C, Harmsen  D, Weniger  T, Rothgänger  J, Hyytiä-Trees  E, Bielaszewska  M, Phylogenetic analysis of enterohemorrhagic Escherichia coli O157, Germany, 1987–2008. Emerg Infect Dis. 2010;16:6106.PubMed
  20. Friedrich  AW, Bielaszewska  M, Zhang  W, Pulz  M, Kuczius  T, Ammon  A, Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J Infect Dis. 2002;185:7484. DOIPubMed
  21. Mellmann  A, Bielaszewska  M, Zimmerhackl  LB, Prager  R, Harmsen  D, Tschäpe  H, Enterohemorrhagic Escherichia coli in human infection: in vivo evolution of a bacterial pathogen. Clin Infect Dis. 2005;41:78592. DOIPubMed
  22. Prager  R, Strutz  U, Fruth  A, Tschäpe  H. Subtyping of pathogenic Escherichia coli strains using flagellar (H)-antigens: serotyping versus fliC polymorphisms. Int J Med Microbiol. 2003;292:47786. DOIPubMed
  23. Sonntag  AK, Prager  R, Bielaszewska  M, Zhang  W, Fruth  A, Tschäpe  H, Phenotypic and genotypic analyses of enterohemorrhagic Escherichia coli O145 strains from patients in Germany. J Clin Microbiol. 2004;42:95462. DOIPubMed
  24. Zhang  Y, Laing  C, Steele  M, Ziebell  K, Johnson  R, Benson  AK, Genome evolution in major Escherichia coli O157:H7 lineages. BMC Genomics. 2007;8:121. DOIPubMed
  25. Wilson  K. Preparation of genomic DNA from bacteria. Curr Protoc Mol Biol. 2001;Chapter 2:Unit 2.4.
  26. Wirth  T, Falush  D, Lan  R, Colles  F, Mensa  P, Wieler  LH, Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol. 2006;60:113651. DOIPubMed
  27. Dugan  KA, Lawrence  HS, Hares  DR, Fisher  CL, Budowle  B. An improved method for post-PCR purification for mtDNA sequence analysis. J Forensic Sci. 2002;47:8118.PubMed
  28. Rump  LV, Strain  EA, Cao  G, Allard  MW, Fischer  M, Brown  EW, Draft genome sequences of six Escherichia coli isolates from the stepwise model emergence of Escherichia coli O157:H7. J Bacteriol. 2011;193:20589. DOIPubMed
  29. Besser  TE, Shaikh  N, Holt  NJ, Tarr  PI, Konkel  ME, Malik-Kale  P, Greater diversity of Shiga toxin–encoding bacteriophage insertion sites among Escherichia coli O157:H7 isolates from cattle than in those from humans. Appl Environ Microbiol. 2007;73:6719. DOIPubMed
  30. Bielaszewska  M, Prager  R, Zhang  W, Friedrich  AW, Mellmann  A, Tschäpe  H, Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM. Appl Environ Microbiol. 2006;72:19009. DOIPubMed
  31. García-Sánchez  A, Sanchez  S, Rubio  R, Pereira  G, Alonso  JM, Hermoso de Mendoza  J, Presence of Shiga toxin–producing E. coli O157:H7 in a survey of wild artiodactyls. Vet Microbiol. 2007;121:3737. DOIPubMed
  32. Díaz  S, Vidal  D, Herrera-Leon  S, Sanchez  S. Sorbitol-fermenting, β-glucuronidase–positive, Shiga toxin–negative Escherichia coli O157:H7 in free-ranging red deer in south-central Spain. Foodborne Pathog Dis. 2011;8:13135. DOIPubMed
  33. Lacher  DW, Steinsland  H, Blank  TE, Donnenberg  MS, Whittam  TS. Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling. J Bacteriol. 2007;189:34250. DOIPubMed
  34. Bielaszewska  M, Köck  R, Friedrich  AW, von Eiff  C, Zimmerhackl  LB, Karch  H, Shiga toxin–mediated hemolytic uremic syndrome: time to change the diagnostic paradigm? PLoS ONE. 2007;2:e1024. DOIPubMed
  35. Mellmann  A, Lu  S, Karch  H, Xu  J, Harmsen  D, Schmidt  MA, Recycling of Shiga toxin 2 genes in sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM. Appl Environ Microbiol. 2008;74:6772. DOIPubMed
  36. Manning  SD, Motiwala  AS, Springman  AC, Qi  W, Lacher  DW, Ouellette  LM, Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaks. Proc Natl Acad Sci U S A. 2008;105:486873. DOIPubMed
  37. Zhang  W, Qi  W, Albert  TJ, Motiwala  AS, Alland  D, Hyytiä-Trees  EK, Probing genomic diversity and evolution of Escherichia coli O157 by single nucleotide polymorphisms. Genome Res. 2006;16:75767. DOIPubMed

Main Article

Page created: March 19, 2012
Page updated: March 19, 2012
Page reviewed: March 19, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external