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Volume 18, Number 5—May 2012

Characterization of Virulent West Nile Virus Kunjin Strain, Australia, 2011

Melinda J. Frost1, Jing Zhang1, Judith H. Edmonds1, Natalie A. Prow1, Xingnian Gu, Rodney Davis, Christine Hornitzky, Kathleen E. Arzey, Deborah Finlaison, Paul Hick, Andrew Read, Jody Hobson-Peters, Fiona J. May, Stephen L. Doggett, John Haniotis, Richard C. Russell, Roy A. Hall2, Alexander A. Khromykh2, and Peter D. Kirkland2Comments to Author 
Author affiliations: Elizabeth Macarthur Agriculture Institute, Menangle, New South Wales, Australia (M.J. Frost, J. Zhang, X. Gu, R. Davis, C. Hornitzky, K.E. Arzey, D. Finlaison, P. Hick, A. Read, P.D. Kirkland); The University of Queensland, St Lucia, Queensland, Australia (J.H. Edmonds, N.A Prow, J. Hobson-Peters, F.J. May, R.A. Hall, A. A. Khromykh); University of Sydney and Westmead Hospital, Westmead, New South Wales, Australia (S.L. Doggett, J. Haniotis, R.C. Russell)

Main Article

Table 2

Binding pattern of monoclonal antibodies to the viral antigens of 3 WNV strains in fixed-cell ELISA, Australia, 2011*

Virus Monoclonal antibody, by specificity
  WNV group
  Unglycosylated WNV E protein
  Glycosylated WNV E protein
4G4, anti-NS1 4G2, anti-E 2B2, anti-E 3.91D, anti-E 10A1, anti-E 5H1, anti-NS5 10C6, anti-NS1
3.101C 17D7
WNVNSW2011 + +   + +   +   +  
WNVKUN + +   + +   + + +    
WNVNY99 + +   + +    −   +  

*WNV, West Nile virus; KUN, Kunjin; E, envelope; MVEV, Murray Valley encephalitis virus; NS, nonstructural protein; NS, nonstructural protein; NSW, New South Wales; + positive; –, negative; NY, New York.
†Prototype WNVKUN strain MRM-61C.
‡North American WNV strain.

Main Article

1These authors contributed equally to the major technical aspects of this research.

2These authors served as joint senior authors.

Page created: April 12, 2012
Page updated: April 12, 2012
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