Volume 18, Number 8—August 2012
Letter
Identification of Cause of Posttransplant Cachexia by PCR
Table A1
Approach used to determine the cause of posttransplant cachexia in a patient*
Pathogen | Sequence target | Primers, 5′ → 3′ | Probes/identification |
---|---|---|---|
Molecular tool to detect and identify Tropheryma whipplei | |||
Real-time PCR | |||
T. whipplei | Repeated sequence | Twhi2F: TGAGGATGTATCTGTGTATGGGACA | 6-FAM-GAGAGATGGGGTGCAGGACAGGG-TAMRA |
Twhi2R: TCCTGTTACAAGCAGTACAAAACAAA | |||
T. whipplei | Repeated sequence | Twhi3F: TTGTGTATTTGGTATTAGATGAAACAG | 6-FAM-GGGATAGAGCAGGAGGTGTCTGTCTGG-TAMRA |
Twhi3R: CCCTACAATATGAAACAGCCTTTG | |||
Molecular tools to detect and identify Mycobacterium spp. | |||
Step 1: Real-time PCR | |||
Mycobacterium spp. | ITS | ITSd: GGGTGGGGTGTGGTGTTTGA ITSr: CAAGGCATCCACCATGCGC | 6-FAM-TGGATAGTGGTTGCGAGCATC-TAMRA |
M. tuberculosis | ITS | ITSd: GGGTGGGGTGTGGTGTTTGA ITSr: CAAGGCATCCACCATGCGC | 6-FAM-GCTAGCCGGCAGCGTATCCAT-TAMRA |
M. avium | ITS | ITSd: GGGTGGGGTGTGGTGTTTGA ITSr: CAAGGCATCCACCATGCGC | 6-FAM-GGCCGGCGTTCATCGAAAT-Mgb |
Step 2: Classical PCR | |||
Mycobacterium spp. | rpoB | MycoF: GGCAAGGTCACCCCGAAGGG MycoR: AGCGGCTGCTGGGTGATCATC | Sequencing |
Housekeeping gene | β-actin | ActinF: CATGCCATCCTGCATCTGGA ActinR: CCGTGGCCATCTCTTGCTCG | 6-FAM-CGGGAAATCGTGCGTGACATTAAG-TAMRA |
*ITS, internal transcribed spacer; rpoB, RNA polymerase B.
Page created: July 18, 2012
Page updated: July 18, 2012
Page reviewed: July 18, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.