Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 18, Number 8—August 2012
Letter

Identification of Cause of Posttransplant Cachexia by PCR

Joelle GuitardComments to Author , Sophie Edouard, Hubert Lepidi, Christine Segonds, Marion Grare, Marie-Laure Ranty-Quintyn, Isabelle Rouquette, Olivier Cointault, Lionel Rostaing, Nassim Kamar, and Florence Fenollar
Author affiliations: Centre Hospitalier Universitaire Rangueil, Toulouse, France (J. Guitard, C. Segonds, M. Grare, M.-L. Ranty-Quintyn, I. Rouquette, O. Cointault, L. Rostaing, N. Kamar); Université Aix-Marseille, Marseille, France (S. Edouard, H. Lepidi, F. Fenollar); Pôle de Maladies Infectieuses, Marseille (S. Edouard, F. Fenollar); and Université Paul Sabatier, Toulouse (L. Rostaing, N. Kamar)

Main Article

Table A1

Approach used to determine the cause of posttransplant cachexia in a patient*

Pathogen Sequence target Primers, 5′ → 3′ Probes/identification
Molecular tool to detect and identify Tropheryma whipplei
Real-time PCR
T. whipplei Repeated sequence Twhi2F: TGAGGATGTATCTGTGTATGGGACA 6-FAM-GAGAGATGGGGTGCAGGACAGGG-TAMRA
Twhi2R: TCCTGTTACAAGCAGTACAAAACAAA
T. whipplei Repeated sequence Twhi3F: TTGTGTATTTGGTATTAGATGAAACAG 6-FAM-GGGATAGAGCAGGAGGTGTCTGTCTGG-TAMRA
Twhi3R:
CCCTACAATATGAAACAGCCTTTG
Molecular tools to detect and identify Mycobacterium spp.
Step 1: Real-time PCR
Mycobacterium spp. ITS ITSd: GGGTGGGGTGTGGTGTTTGA
ITSr: CAAGGCATCCACCATGCGC 6-FAM-TGGATAGTGGTTGCGAGCATC-TAMRA
M. tuberculosis ITS ITSd: GGGTGGGGTGTGGTGTTTGA
ITSr: CAAGGCATCCACCATGCGC 6-FAM-GCTAGCCGGCAGCGTATCCAT-TAMRA
M. avium ITS ITSd: GGGTGGGGTGTGGTGTTTGA
ITSr: CAAGGCATCCACCATGCGC 6-FAM-GGCCGGCGTTCATCGAAAT-Mgb
Step 2: Classical PCR
Mycobacterium spp. rpoB MycoF: GGCAAGGTCACCCCGAAGGG
MycoR: AGCGGCTGCTGGGTGATCATC Sequencing
Housekeeping gene β-actin ActinF: CATGCCATCCTGCATCTGGA
ActinR: CCGTGGCCATCTCTTGCTCG 6-FAM-CGGGAAATCGTGCGTGACATTAAG-TAMRA

*ITS, internal transcribed spacer; rpoB, RNA polymerase B.

Main Article

Page created: July 18, 2012
Page updated: July 18, 2012
Page reviewed: July 18, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external