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Volume 19, Number 11—November 2013
Research

Atypical Scrapie Prions from Sheep and Lack of Disease in Transgenic Mice Overexpressing Human Prion Protein

Jonathan D.F. WadsworthComments to Author , Susan Joiner, Jacqueline M. Linehan, Anne Balkema-Buschmann, John Spiropoulos, Marion M. Simmons, Peter C. Griffiths, Martin H. Groschup, James Hope, Sebastian Brandner, Emmanuel A. Asante, and John Collinge
Author affiliations: University College London, London, UK (J.D.F Wadsworth, S. Joiner, J.M. Linehan, S. Brandner, E.A. Asante, J. Collinge); Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany (A. Anne Balkema-Buschmann, M.H. Groschup); Animal Health and Veterinary Laboratories Agency, Addlestone, UK (J. Spiropoulos, M.M. Simmons, P.C. Griffiths, J. Hope)

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Table 2

Survival times of transgenic human PrP mice after inoculation of ovine prions*

Source code Prion agent Transmission data
129MM Tg35c mice
 129VV Tg152c mice
Attack rate† Survival, d‡ Attack rate† Survival, d‡
AHVLA/SE 1919/0077 Classical scrapie 0/20 551, 551, 583, 615–666 (17) 0/16 301, 344, 344, 364, 386, 428, 475, 519, 540, 543, 600–601 (6)
AHVLA/SE 1919/0080 Classical scrapie 0/19 580, 586, 586, 620–666 (16) 0/14 211, 336, 364, 364, 379, 519, 601–602 (8)
FLI 1/06 Classical scrapie 0/15 426, 475, 628–728 (13) 0/17 364, 497, 498, 517, 547, 559, 571, 595, 603–673 (9)
FLI 83/04 Classical scrapie 0/15 270, 307, 311, 335, 349, 353,
635–672 (9) 0/16 227, 300, 335, 440, 479, 510,
600–650 (10)
FLI 107/04 Classical scrapie 0/17 382, 382, 459, 573, 574, 578, 606–636 (11) 0/13 227, 228, 476, 606–706 (10)
AHVLA/SE 1850/0001 Atypical scrapie 0/18 213, 332, 437, 537, 537,
621–656 (13) 0/18 255, 318, 385, 397, 402, 403, 452, 453, 493, 518, 528, 538, 543, 552, 633–647 (4)
AHVLA/SE 1850/0009 Atypical scrapie 0/18 440, 606–635 (17) 0/15 293, 334, 403, 404, 419, 420, 426, 444, 584, 637–651 (6)
FLI S7/06 Atypical scrapie 0/16 498, 610–659 (15) 0/14 539, 545, 630–673 (12)
FLI 14/06 Atypical scrapie 0/18 538, 540, 545, 572, 601–728 (14) 0/15 313, 363, 489, 510, 592, 602–673 (10)
FLI 26/06 Atypical scrapie 0/14 547, 553, 643–659 (12) 0/14 435, 446, 554, 571, 608–673 (10)
AHVLA/SE 1929/0877 Ovine BSE 0/16 315, 316, 348, 459, 557, 581, 620–659 (10) 0/18 358, 363, 369, 382, 385, 440, 468, 476, 532, 550, 574, 600–602 (7)
AHVLA/SE 1945/0032 2nd Passage ovine BSE 1/19 337, 337, 434, 472, 517, 524, 616–661 (13) 0/17 331, 331, 381, 386, 388, 388, 525, 527, 542, 562, 603–608 (7)

*PrP, prion protein; AHVLA, Animal Health and Veterinary Laboratories Agency; FLI, Friedrich-Loeffler-Institut; BSE, bovine spongiform encephalopathy.
†All mice were inoculated with 30 μL of 1% (w/v) brain homogenate. Attack rate is defined as the total number of clinically affected and subclinically infected mice as a proportion of the number of inoculated mice. Subclinical prion infection was assessed by immunohistochemical examination of brain for abnormal PrP deposition and for recipients of AHVLA inocula by sodium phosphotungstic acid precipitation of 250 μL 10% brain homogenate and analysis for PrPSc by proteinase K digestion and immunoblotting.
‡The interval between inoculation and culling because of intercurrent illness, senescence, or termination of the experiment in days. Death dates of individual mice are shown together with the range for mice surviving beyond 600 d with the number of mice in this range shown in parentheses. Mice culled with postinoculation periods of ≤200 d due to intercurrent illness (all confirmed negative for prion infection) were not included in calculating attack rates.

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Page created: October 31, 2013
Page updated: October 31, 2013
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