Volume 19, Number 5—May 2013
Research
Foodborne Transmission of Bovine Spongiform Encephalopathy to Nonhuman Primates
Table 2
Macaque | Sex | BSE dose, g | Euthanized, y postinoculation | 14-3-3p–positive CSF | PrPres pattern (brain) | PrPres pattern (spinal cord C1–T12) | PrPres pattern (spinal cord L1–L4) |
---|---|---|---|---|---|---|---|
Group I (clinically infected)† | |||||||
S1 | F | 5 | 4.3 | Yes | 2B | 2B | 2B |
S2 | F | 5 | 4.6 | Yes | 2B | 2B | 2B |
S3 | M | 5 | 4.7 | Yes | 2B | 2B | 2B |
S4 | F | 5 | 4.8 | Yes | 2B | 2B | 2B |
S5 | F | 5 | 5.2 | Yes | 2B | 2B | 2B |
S6‡ | F | 16 | 3.7 | Yes | 2B | 2B | 2B |
S7 | F | 16 | 4.5 | Yes | 2B | 2B | 2B |
S8 |
F |
16 |
5.3 |
Yes |
2B |
2B |
2B |
Group II (preclinical)§ | |||||||
S9 | M | 5 | 1.0 | No | Neg | Neg | Neg |
S10 | M | 5 | 1.0 | No | Neg | Neg | Neg |
S11 | M | 5 | 3.0 | No | Neg | Neg | Non-2B |
S12 | M | 5 | 3.0 | No | Neg | Neg | Non-2B |
S13 | M | 5 | 3,9 | No | Neg | Th7–10+ | Neg |
S14 | F | 5 | 4,1 | No | Neg | Neg | Non-2B |
S15 |
F |
5 |
5.0 |
No |
Neg |
Neg |
Non-2B |
Group III (preclinical)¶ | |||||||
C1 | F | 8 | 6.5 | No | Neg | Neg | Non-2B |
C2 | F | 10 | 6.5 | No | Neg | Neg | Non-2B |
C3 |
F |
16 |
8.8 |
No |
Neg |
Neg |
Neg |
Group IV (controls)# | |||||||
M1 | F | 5 | 2.0 | No | Neg | Neg | Neg |
M2 | F | 5 | 5.0 | No | Neg | Neg | Neg |
M3 | F | 16 | 6.0 | No | Neg | Neg | Neg |
M4 | F | 16 | 6.0 | No | Neg | Neg | Neg |
M5 | F | 16 | 6.0 | No | Neg | Neg | Neg |
M6 | F | 16 | 6.0 | No | Neg | Neg | Neg |
M7 | F | 0.05 | 6.0 | No | Neg | Neg | Neg |
M8 | F | 0.05 | 6.0 | No | Neg | Neg | Neg |
*BSE, bovine spongiform encephalopathy; PrPres, proteinase-resistant prion protein; CSF, cerebrospinal fluid; C, cervical; T, thoracic; L, lumbar; neg, negative.
†Received 1 BSE dose, observed until onset of clinical signs.
‡Macaque S6 had a highly stimulated immune system on the day of oral exposure and thereafter (reason unknown) and had the highest postmortem levels of proteinease-resistant prion protein (PrPres) in spleen and other lymphoreticular tissues of all examined macaques (data not shown).
§Animals received 1 BSE dose and were euthanized at regular intervals during incubation period (all animals had PrPres -positive non–central nervous system tissue).
¶Cumulative BSE dose, euthanized.
#Exposed to mock (non–BSE-infected) bovine brain material and euthanized during aging to act as age-matched controls.