Table 4

Antigenic and Molecular Characterization of Avian Influenza A(H9N2) Viruses, Bangladesh

Karthik Shanmuganatham, Mohammed M. Feeroz, Lisa Jones-Engel, Gavin J.D. Smith, Mathieu Fourment, David Walker, Laura McClenaghan, S.M. Rabiul Alam, M. Kamrul Hasan, Patrick Seiler, John Franks, Angie Danner, Subrata Barman, Pamela McKenzie, Scott Krauss, Richard J. Webby, and Robert G. Webster

Author affiliations: St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (K. Shanmuganatham, D. Walker, L. McClenaghan, P. Seiler, J. Franks, A. Danner, S. Barman, P. McKenzie, S. Krauss, R.J. Webby, R.G. Webster); Jahangirnagar University, Dhaka, Bangladesh (M.M. Feeroz, S.M.R. Alam, M.K. Hasan); University of Washington, Seattle, Washington, USA (L. Jones-Engel); Duke-NUS Graduate Medical School, Singapore (G.J.D. Smith, M. Fourment); Duke Global Health Institute, Durham, North Carolina, USA (G.J.D. Smith)

Main Article

HA, NA, and M gene substitutions that favor replication in mammals in avian influenza A(H9N2) viruses isolated from poultry in Bangladesh during 2008–2011*†

Residues and isolates	HA						NA		M1	M2 drug resistance
Residues and isolates	HA RBS				HA1/HA2	Glyco site	HB site		15	16	20	28	55	26	31
Residues
H9N2 numbering	166	191	234	399		218	372	403
H3 numbering	158	183	226	391
Isolates
A/Env/BD/907/2009	N	H	L	K	KSSR	No	A	W	I	V	S	F	F	L	N
A/Env/BD1041/2009	S	H	Q	K	ASDR	NRTF	S	W	V	E	S	I	L	L	S
A/Ck/BD/2075/2009	N	H	L	K	KSSR	No	A	W	I	V	S	F	F	L	S
A/Env/BD/8202/2010	N	H	L	K	KSSR	No	A	W	I	V	N	F	F	F	N
A/Ck/BD/8996/2010	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Ck/BD/9334/2010	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/10234/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/10306/2011	D	H	Q	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Ck/BD/10450/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Ck/BD/10897/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Ck/BD/11154/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/11173/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/11309/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/11315/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/12116/2011	N	H	L	K	KSKR	No	ND	ND	I	V	S	F	F	L	N
A/Env/BD/12119/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N
A/Env/BD/13916/2011	N	H	L	K	KSSR	No	A	R	I	V	S	F	F	L	N
A/Ck/BD/13962/2011	N	H	L	K	KSKR	No	A	W	I	V	S	F	F	L	N

*A total of 31 unique substitutions were identified in 18 H9 isolates. Isolates 8202 (M2 adamantine-like drug resistance mutation), 10306 (HA158D) and13916 (NA403R) contain unique substitution not seen in most isolates. Boldface indicates key amino acid substitutions implicated in mammalian transmission. HA, hemagglutinin; NA, neuraminidase; M, matrix; RBS, receptor-binding site; glyco, glycosylation; HB, hemabsorbing; ND, analysis not done because sequences were not available.
†Amino acids: A, alanine; D, aspartic acid; E, glutamic acid; H, histidine; I, isoleucine; K, lysine; L, leucine; N, asparagine; Q, glutamine; R, arginine; S, serine; V, valine.

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Page created: August 20, 2013

Page updated: August 20, 2013

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