Volume 19, Number 9—September 2013
Research
Antigenic and Molecular Characterization of Avian Influenza A(H9N2) Viruses, Bangladesh
Table 5
NS, NP, PA, PB1, and PB2 gene substitutions that favor replication in mammals in avian influenza A(H9N2) viruses isolated from poultry in Bangladesh during 2008–2011*†
Isolate | NS |
NP |
PA |
PA-X | PB1 |
PB1-F2 |
PB2 |
||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NS1 (227) | PL motif | 33 | 109 | 214 | 313 | 372 | 57 | 409 | 13 | 336 | 375 | 66 | 68 | 73 | 82 | 81 | 318 | ||||||
A/Env/BD/907/2009 | K | KSEV | V | I | K | F | D | R | S | Yes | P | V | N | N | T | K | S | T | K | ||||
A/Env/BD1041/2009 | E | ESEV | V | I | R | F | E | R | S | ND | ND | ND | ND | ND | ND | ND | T | R | |||||
A/Ck/BD/2075/2009 | K | KSEV | V | I | R | F | D | R | S | P | V | T | N | T | K | L | T | K | |||||
A/Env/BD/8202/2010 | K | KPEV | V | V | K | Y | D | R | S | P | V | S | N | T | K | L | I | K | |||||
A/Ck/BD/8996/2010 | K | KSEV | V | V | K | F | D | R | N | P | V | S | N | T | K | L | I | K | |||||
A/Ck/BD/9334/2010 | ND | ND | I | I | K | F | D | R | S | P | V | N | N | T | K | L | T | K | |||||
A/Env/BD/10234/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | S | N | T | K | L | I | K | |||||
A/Env/BD/10306/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | N | N | I | K | L | I | K | |||||
A/Ck/BD/10450/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | N | N | T | K | L | T | K | |||||
A/Ck/BD/10897/2011 | K | KSEV | V | I | K | F | D | R | S | P | V | S | N | T | K | L | T | K | |||||
A/Ck/BD/11154/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | N | N | T | K | L | T | K | |||||
A/Env/BD/11173/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | N | N | T | K | L | I | K | |||||
A/Env/BD/11309/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | N | N | T | K | L | ND | ND | |||||
A/Env/BD/11315/2011 | K | KSEV | V | V | K | F | D | R | S | P | V | N | N | T | K | L | I | K | |||||
A/Env/BD/12116/2011 | K | KSEV | V | V | K | F | D | ND | ND | P | V | N | N | T | K | L | I | K | |||||
A/Env/BD/12119/2011 | K | KSEV | V | V | K | F | D | ND | ND | P | V | N | N | T | K | L | I | K | |||||
A/Env/BD/13916/2011 | K | KSEV | V | I | K | F | D | ND | ND | P | I | N | N | T | K | L | T | K | |||||
A/Ck/BD/13962/2011 | K | KSEV | V | V | K | F | D | ND | ND | P | V | N | S | T | R | L | I | K |
*A total of 31 unique substitutions were identified in 18 H9 isolates. Isolates 907 (PB1-F2, 82S), 8202 (NP-313Y), 10306 (PB1-F2 68I), 10897 (PB1375S), 13916 (PB1336I), and 13962(PB1-F2 66S/82R) contain unique substitution not seen in most isolates. Boldface indicates key amino acid substitutions implicated in mammalian transmission. NS, nonstructural; NP, nucleoprotein; PA and PB, polymerase genes; ND, analysis not done because sequences were not available.
†Amino acids: D, aspartic acid; E, glutamic acid; I, isoleucine; K, lysine; L, leucine; N, asparagine; R, arginine; S, serine; T, threonine; V, valine.