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Volume 2, Number 3—July 1996


Application of Molecular Techniques
to the Diagnosis of Microsporidial Infection

Daniel P. FedorkoComments to Author  and Yasmine M. Hijazi
Author affiliations: National Institutes of Health, Bethesda, Maryland, USA

Main Article

Table 2

SSU-rRNA PCR primer pairs for the diagnosis of microsporidial infection

Primer pair (no. nucleotides) Primer designation Organisms amplified Amplicon size in base pairs Source of Target References
5'-CACCAGGTTGATTCTGCCTGAC-3' (22) V1 Enterocytozoon bieneusi 348 Biopsied tissue Duodenal aspirates 33, 41 31
5'-ACTCAGGTGTTATACTCACGTC-3' (22) EB450 Encephalitozoon hellem Cultured organisms 33
5'-GAAACTTGTCCACTCCTTACG-3' (21) EBIEF1 E.bieneusi 607 Bile 43
5'-CCATGCACCACTCCTGCCATT-3' (21) EBIER1 Duodenal aspirates
5'-TGAGAAGTAAGATGTTTAGCA-3' (21) E.hellem 547 Cultured organisms 39
5'-ATGAGAAGTGATGTGTGTGCG-3' (21) Encephalitozoon cuniculi 549 Cultured organisms 39, 40
5'-CACCAGGTTGATTCTGCCTGAC-3' (22) V1 Encephalitozoon intestinalis 370 Biopsied tissue 31
5'-CACCAGGTTGATTCTGCCTGAC-3' (22) PMP1* E.bieneusi 250 Stool 42
5'-CCTCTCCGGAACCAAACCTG-3' (21) PMP2 E.cuniculli 268 Cultured organisms
E.intestinalis 270 Stool
E.hellem 279 Cultured organisms

*The nucleotide sequence of primer PMP1 is identical to that of primer V1.

Main Article