Volume 20, Number 1—January 2014
Research
Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast
Table 1
Demographic characteristics and diagnostic tests performed with specimens from meningitis cohorts, Uganda and South Africa*
Characteristic |
2006–2009, Retrospective |
2010–2012, Prospective* |
||
---|---|---|---|---|
Demographic |
Kampala, Uganda |
Kampala, Uganda |
Mbarara, Uganda |
Cape Town, South Africa |
Location | ||||
No. persons | 299 | 354 | 142 | 37 |
Mean ± SD age, y | 36 ± 8 | 35 ± 9 | 35 ± 11 | 37 ± 10 |
Male sex, no. (%) | 168 (56) | 174 (49) | 87 (61) | 22 (59) |
CD4 cell count/μL median (IQR) | 19 (7–38) | 16 (7–69) | 36 (14–74) | 65 (43–97) |
Diagnostic tests performed, no. | ||||
CSF | ||||
Quantitative culture | 282 | 345 | 142 | 37 |
Latex agglutination | 279 | 345 | 142 | 37 |
India ink microscopy | 276 | 350 | 142 | 37 |
LFA | 197 | 291 | 142 | 36 |
Serum | ||||
Latex agglutination | 85 | 34 | NA | NA |
LFA | NA | 274 | 49 | 23 |
Plasma | ||||
LFA | 60 | NA | NA | NA |
Urine | ||||
LFA | NA | 185 | 51 | NA |
*IQR, interquartile range; CSF, cerebrospinal fluid; LFA, lateral flow immunochromatographic assay; NA, not available.
†Prospective testing with LFA because point-of-care began in April 2011. Eighty LFAs were conducted on cryopreserved samples.