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Volume 20, Number 1—January 2014
Research

Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast

David R. BoulwareComments to Author , Melissa A. Rolfes, Radha Rajasingham, Maximilian von Hohenberg, Zhenpeng Qin, Kabanda Taseera, Charlotte Schutz, Richard Kwizera, Elissa K. Butler, Graeme Meintjes, Conrad Muzoora, John C. Bischof, and David B. Meya
Author affiliations: University of Minnesota, Minneapolis, Minnesota, USA (D.R. Boulware, M.A. Rolfes, R. Rajasingham, M. von Hohenberg, Z. Qin, E.K. Butler, J.C. Bischof, D.B. Meya); Makerere University, Kampala, Uganda (R. Rajasingham, R.K. Kwizera, D.B. Meya); Mbarara University of Science and Technology, Mbarara, Uganda (K. Taseera, C. Muzoora); G.F. Jooste Hospital, Cape Town, South Africa (C. Schutz, G. Meintjes); University of Cape Town, Cape Town (C. Schutz, G. Meintjes)

Main Article

Table 1

Demographic characteristics and diagnostic tests performed with specimens from meningitis cohorts, Uganda and South Africa*

Characteristic
 2006–2009, Retrospective
 2010–2012, Prospective*
Demographic
 Kampala, Uganda
 Kampala, Uganda
 Mbarara, Uganda
 Cape Town, South Africa
Location
No. persons 299 354 142 37
Mean ± SD age, y 36 ± 8 35 ± 9 35 ± 11 37 ± 10
Male sex, no. (%) 168 (56) 174 (49) 87 (61) 22 (59)
CD4 cell count/μL median (IQR) 19 (7–38) 16 (7–69) 36 (14–74) 65 (43–97)
Diagnostic tests performed, no.
CSF
Quantitative culture 282 345 142 37
Latex agglutination 279 345 142 37
India ink microscopy 276 350 142 37
LFA 197 291 142 36
Serum
Latex agglutination 85 34 NA NA
LFA NA 274 49 23
Plasma
LFA 60 NA NA NA
Urine
LFA NA 185 51 NA

*IQR, interquartile range; CSF, cerebrospinal fluid; LFA, lateral flow immunochromatographic assay; NA, not available.
†Prospective testing with LFA because point-of-care began in April 2011. Eighty LFAs were conducted on cryopreserved samples.

Main Article

Page created: January 03, 2014
Page updated: January 03, 2014
Page reviewed: January 03, 2014
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