Figure 4. Chromosome location of the recombination region located upstream of the penicillin-binding protein 2x (pbp2x) gene of Streptococcus pneumoniae (A) and antimicrobial drug resistance determinants, capsular loci of the putative donor, recipient, and recombinant strains (B). In the genomic region upstream of pbp2x (SPG_0305), genes have been named as in the G54 genome (ST63) (GenBank accession no. CP001015). Number of polymorphic sites located either within the genes (inserted into the arrows or into black boxes) or in their intergenic regions (gray boxes) is shown. The approximate location of the recombination event is indicated by the dotted box. Amplified and sequenced fragments are indicated in relationship to primer pair designations (Table). MICs for penicillin (Pen) are shown.