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Volume 20, Number 3—March 2014

Research

High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks

Hervé Tettelin, Rebecca M. Davidson, Sonia Agrawal, Moira L. Aitken, Shamira Shallom, Nabeeh A. Hasan, Michael Strong, Vinicius Calado Nogueira de Moura, Mary Ann De Groote, Rafael S. Duarte, Erin Hine, Sushma Parankush, Qi Su, Sean C. Daugherty, Claire M. Fraser, Barbara A. Brown-Elliott, Richard J. Wallace, Steven M. Holland, Elizabeth P. Sampaio, Kenneth N. Olivier, Mary Jackson, and Adrian M. ZelaznyComments to Author 
Author affiliations: University of Maryland School of Medicine, Baltimore, Maryland, USA (H. Tettelin, S. Agrawal, E. Hine, S. Parankush, Q. Su, S.C. Daugherty, C.M. Fraser); National Jewish Health, Denver, Colorado, USA (R.M. Davidson, N.A. Hasan, M. Strong); University of Washington, Seattle, Washington, USA (M.L. Aitken); National Institutes of Health, Bethesda, Maryland, USA (S. Shallom, S.M. Holland, E.P. Sampaio, K.N. Olivier, A.M. Zelazny); University of Colorado Denver, Aurora, Colorado, USA (N.A. Hasan, M. Strong); Colorado State University, Fort Collins, Colorado, USA (V. Calado Nogueira de Moura, M.A. De Groote, M. Jackson); Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil (R.S. Duarte); University of Texas Health Northeast, Tyler, Texas, USA (B.A. Brown-Elliott, R.J. Wallace Jr.)

Main Article

Figure 1

Neighbor-joining phylogenetic tree based on whole-genome multiple alignment of 24 Mycobacterium abscessus group genomes. Genomes in Table 1 were aligned by using Mugsy (22), core segments of the alignment were identified by using Phylomark (23), and resulting concatenated nucleotide sequences were used for construction of the midpoint-rooted neighbor-joining phylogenetic tree by using MEGA (24). Strains from an outbreak of M. abscessus subsp. massiliense infections at a cystic fibrosis center in

Figure 1. Neighbor-joining phylogenetic tree based on whole-genome multiple alignment of 24 Mycobacterium abscessus group genomesGenomes in Table 1 were aligned by using Mugsy (22), core segments of the alignment were identified by using Phylomark (23), and resulting concatenated nucleotide sequences were used for construction of the midpoint-rooted neighbor-joining phylogenetic tree by using MEGA (24)Strains from an outbreak of Mabscessus subspmassiliense infections at a cystic fibrosis center in Seattle, Washington, USA, are indicated in red; strains from an outbreak of Mabscessus subspmassiliense infections at a cystic fibrosis center in Papworth, UK, are indicated in blue (cluster 1) and purple (cluster 2); strains from Brazil are indicated in magenta; and the Mabscessus subspmassiliense type strain is indicated in greenBoostrap values obtained after 100 iterations were ≥97 for all nodes of the tree except 70 for the node separating strain M115 from the outbreak cluster and 40 and 41 for 2 nodes within the Papworth cluster 1 (6)SNPs, single-nucleotide polymorphisms.

Main Article

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Page created: February 19, 2014
Page updated: February 19, 2014
Page reviewed: February 19, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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