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Volume 20, Number 3—March 2014
Research

High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks

Hervé Tettelin, Rebecca M. Davidson, Sonia Agrawal, Moira L. Aitken, Shamira Shallom, Nabeeh A. Hasan, Michael Strong, Vinicius Calado Nogueira de Moura, Mary Ann De Groote, Rafael S. Duarte, Erin Hine, Sushma Parankush, Qi Su, Sean C. Daugherty, Claire M. Fraser, Barbara A. Brown-Elliott, Richard J. Wallace, Steven M. Holland, Elizabeth P. Sampaio, Kenneth N. Olivier, Mary Jackson, and Adrian M. ZelaznyComments to Author 
Author affiliations: University of Maryland School of Medicine, Baltimore, Maryland, USA (H. Tettelin, S. Agrawal, E. Hine, S. Parankush, Q. Su, S.C. Daugherty, C.M. Fraser); National Jewish Health, Denver, Colorado, USA (R.M. Davidson, N.A. Hasan, M. Strong); University of Washington, Seattle, Washington, USA (M.L. Aitken); National Institutes of Health, Bethesda, Maryland, USA (S. Shallom, S.M. Holland, E.P. Sampaio, K.N. Olivier, A.M. Zelazny); University of Colorado Denver, Aurora, Colorado, USA (N.A. Hasan, M. Strong); Colorado State University, Fort Collins, Colorado, USA (V. Calado Nogueira de Moura, M.A. De Groote, M. Jackson); Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil (R.S. Duarte); University of Texas Health Northeast, Tyler, Texas, USA (B.A. Brown-Elliott, R.J. Wallace Jr.)

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Figure 3

Neighbor-joining phylogenetic tree based on 13-target multilocus sequences types from 20 Mycobacterium abscessus subsp. massiliense genomes. Electronic PCR was performed on the M. abscessus subsp. massiliense genomes listed in Table 1 by using primer pairs for 13 housekeeping genes (cya, gdhA, argH, glpK, gnd, murC, pgm, pknA, pta, pur, rpoB, hsp65, and secA1), including new primers designed as part of this study. Nucleotide sequences from each gene were concatenated for each genome and aligned

Figure 3. Neighbor-joining phylogenetic tree based on 13-target multilocus sequences types from 20 Mycobacterium abscessus subspmassiliense genomesElectronic PCR was performed on the Mabscessus subspmassiliense genomes listed in Table 1 by using primer pairs for 13 housekeeping genes (cya, gdhA, argH, glpK, gnd, murC, pgm, pknA, pta, pur, rpoB, hsp65, and secA1), including new primers designed as part of this studyNucleotide sequences from each gene were concatenated for each genome and aligned by using ClustalW (31), and the core alignment was used for construction of a midpoint-rooted neighbor-joining phylogenetic tree by using MEGA (24)Strains from an outbreak of Mabscessus subspmassiliense infections at a cystic fibrosis center in Seattle, Washington, USA, are indicated in red; strains from an outbreak of Mabscessus subspmassiliense infections at a cystic fibrosis center in Papworth, UK, are indicated in blue (cluster 1) and purple (cluster 2); strains from Brazil are indicated in magenta; and the Mabscessus subspmassiliense type strain is indicated in greenThe longer branch length for Papworth isolate 12c was caused by low-quality nucleotides (single-nucleotide polymorphisms [SNPs]) located at the edge of Velvet contigs.

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Page updated: February 19, 2014
Page reviewed: February 19, 2014
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