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Volume 20, Number 4—April 2014
Research

Novel Betacoronavirus in Dromedaries of the Middle East, 2013

Patrick C.Y. Woo1Comments to Author , Susanna K.P. Lau1, Ulrich Wernery, Emily Y.M. Wong, Alan K.L. Tsang, Bobby Johnson, Cyril C.Y. Yip, Candy C.Y. Lau, Saritha Sivakumar, Jian-Piao Cai, Rachel Y.Y. Fan, Kwok-Hung Chan, Ringu Mareena, and Kwok-Yung Yuen
Author affiliations: The University of Hong Kong, Hong Kong, China (P.C.Y. Woo, S.K.P. Lau, E.Y.M. Wong, A.K.L. Tsang, C.C.Y. Yip, C.C.Y. Lau, J.-P. Cai, R.Y.Y. Fan, K.H. Chan, K.-Y. Yuen); Central Veterinary Research Laboratory, Dubai, United Arab Emirates (U. Wernery, B. Johnson, S. Sivakumar, R. Mareena)

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Figure 6

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis of a novel coronavirus, dromedary camel coronavirus UAE-HKU23, discovered in dromedaries of the Middle East, 2013.Nucleocapsid protein was expressed in Escherichia coli. M, protein molecular-mass marker; kDa, kilodaltons. Lanes: 1, non-induced crude E. coli cell lysate; 2, induced crude E. coli cell lysate of DcCoV UAE-HKU23 nucleocapsid protein; 3, purified recombinant DcCoV UAE-HKU23 nucleocapsid protein; 4, dr

Figure 6. . . . . . Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis of a novel coronavirus, dromedary camel coronavirus UAE-HKU23, discovered in dromedaries of the Middle East, 2013.Nucleocapsid protein was expressed in Escherichia coli. M, protein molecular-mass marker; kDa, kilodaltons. Lanes: 1, non-induced crude E. coli cell lysate; 2, induced crude E. coli cell lysate of DcCoV UAE-HKU23 nucleocapsid protein; 3, purified recombinant DcCoV UAE-HKU23 nucleocapsid protein; 4, dromedary camel serum sample strongly positive for antibody against nucleocapsid protein; 5, dromedary camel serum sample moderately positive for antibody against nucleocapsid protein; 6 and 7: dromedary camel serum sample negative for antibody against nucleocapsid protein.

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1These authors contributed equally to this article.

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