Volume 20, Number 7—July 2014
Dispatch
Detection and Genetic Characterization of Deltacoronavirus in Pigs, Ohio, USA, 2014
Table 2
Oligonucleotide primers used for amplification of the porcine coronavirus HKU15 genomic fragments by reverse transcription PCR, Ohio, USA, 2014
| Primer identification | Sequence (5′→3′) | Nucleotide position* | Fragment |
|---|---|---|---|
| Dcor-1-F | ACATGGGGACTAAAGATAAAAATTATAGC | 1–29 | 1 |
| DCor-1610-R |
AGACGGGCCAATTTTGACCG |
1591–1610 |
|
| DCor-1481-F | TGATGATGTT CTGCTAGCCT | 1481–1500 | 2 |
| DCor-3300-R |
GCTCATCGCCTACATCAGTA |
3281–3300 |
|
| DCor-3091-F | CGGATTTAAAACCACAGACT | 3091–3110 | 3 |
| Dcor-4860-R |
ACGACTTTACGAGGATGAAT |
4841–4860 |
|
| DCor-4741-F | CTCCTGTACAGGCCTTACAA | 4741–4760 | 4 |
| DCor-6420-R |
TCACACGTATAGCCTGCTGA |
6401–6420 |
|
| DCor-6291-F | CTCAATGCAGAAGACCAGTC | 6291–6310 | 5 |
| DCor-8041-R |
CAGCTTGGTCTTAAGACTCT |
8041–8060 |
|
| DCor-7920-F | GGTACTGCTTCTGATAAGGAT | 7920–7940 | 6 |
| DCor-9660-R |
TAGGTACAGTTGTGAACCGA |
9641–9660 |
|
| DCor-9541-F | CTCTGCCCATTATCATGCCT | 9541–9560 | 7 |
| DCor-11040-R |
AAAGAGAGGCATTTTGCTGG |
11021–11040 |
|
| DCor-10861-F | ACTTGGACCCTCCTATGCGC | 10861–10880 | 8 |
| DCor-12840-R |
GGCTCAAGATACTTATCTGC |
12821–12840 |
|
| DCor-12721-F | TATGCAGGATGGTGAAGCGG | 12721–12740 | 9 |
| DCor-14400-R |
TCACAATAAATCGCAGTGCC |
14381–14000 |
|
| DCor-14281-F | TGTTACGCAGACTACACATA | 14281–14300 | 10 |
| DCor-16020-R |
TCATAGCCGCAGCGCTTAAA |
16001–16020 |
|
| DCor-15901-F | TGTGGTGTTTAGGCAGGCAA | 15901–15920 | 11 |
| DCor-17760-R |
GTGGCGGTTACGCCTAAACC |
17741–17760 |
|
| DCor-17641-F | CAAACTCTTTGACAATCGCA | 17641–17660 | 12 |
| DCor-19200-R |
GCTAAAGGAGAATAGGTTGGTG |
19179–19200 |
|
| DCor-18981-F | CTGAACATTCATTCTCACCC | 18981–19000 | 13 |
| DCor-20910-R |
GAAGGTGGTGGCATTTGTGG |
20891–20910 |
|
| DCor-20761-F | GTCTTACCGTGTGAAACCCC | 20761–20780 | 14 |
| DCor-22440-R |
AACATCCCACTGAGGAGGTG |
22421–22440 |
|
| DCor-22321-F | TTTTATAACACCACCGCTGC | 22321–22340 | 15 |
| DCor-24004-R |
GGCCATGATAGATTGGTGTC |
23985–24004 |
|
| DCor-23881-F | ATGGTGAGCCTTTACTGCTT | 23881–23900 | 16 |
| DCor-25417-R | TGCTCCATCCCCCCTATAAG | 25398–25417 |
*Positions correspond to porcine coronavirus HKU15-155 strain (GenBank accession no. JQ065043).
1These authors were co–principal investigators.
Page created: June 17, 2014
Page updated: June 17, 2014
Page reviewed: June 17, 2014
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